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全长度转录组测序和比较转录组学揭示了睡眠鱼 (Oxyeleotris lineolata) 阴道发育的基础分子机制
Jiajia Fan1,2, Dongmei Ma1,2, Huaping Zhu1,2
1Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.
Biology
|March 26, 2025
概括
研究人员生成了睡眠鱼的全长转录组,识别了参与性别决定的关键基因和途径. 这提供了宝贵的遗传资源,以提高这种经济重要鱼类的水产养殖产量.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 基因组学就是基因组学.
- 鱼类生物学 鱼类生物学
背景情况:
- 沉睡的鱼 (Oxyeleotris lineolata) 是一种具有经济重要性的水产养殖物种,具有两性两态的生长.
- 了解确定性别的分子基础对于提高水产养殖产量至关重要.
- 有限的基因组和转录基因组数据阻碍了对睡眠鱼的性别确定研究.
研究的目的:
- 为了生成睡眠鱼的全长转录组.
- 为了确定差异表达基因 (DETs) 和涉及淋巴体分化的途径.
- 为睡眠鱼的性别控制繁殖提供基因组资源.
主要方法:
- 长读PacBio异形测序 (Iso-Seq) 用于全长的转录组组装.
- 短读Illumina测序用于卵巢和丸之间的差异基因表达分析.
- 定量PCR (qPCR) 用于验证转录基因数据.
主要成果:
- 产生了49,113个非冗余的全长副本.
- 确定了19102个性别差异表达的转录.
- 卵细胞半转化和阿拉基酸代谢途径在淋巴体分化方面得到了显著的丰富.
结论:
- 这项研究为睡眠鱼提供了宝贵的全长转录组资源.
- 确定了关键的基因和途径,为性别决定机制提供了洞察力.
- 研究结果支持开发性别控制策略,以提高水产养殖产量.
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