斑马鱼中化物暴露对神经行为的影响:评估认知缺陷和益生菌调节
Madalina Ene1, Alexandra Savuca2,3, Alin-Stelian Ciobica1,3,4,5,6
1Department of Biology, Faculty of Biology, "Alexandru Ioan Cuza" University of Iasi, Carol I Avenue, 20A, 700505 Iasi, Romania.
Toxics
|March 26, 2025
概括
益生菌没有显著改善斑马鱼中化 (ZnCl2) 暴露引起的认知缺陷. 然而,益生菌显示出抗焦虑作用,这表明对重金属毒性的部分神经行为益处.
科学领域:
- 环境毒理学环境毒理学
- 神经科学是一个神经科学.
- 微生物学 微生物学
背景情况:
- 污染是一个日益严重的环境问题,对水生生物构成神经毒性风险.
- 过多的会破坏肠-大脑轴,导致认知和行为障碍.
- 益生菌正在研究它们通过肠道微生物群调节减轻神经毒素影响的潜力.
研究的目的:
- 评估益生菌在改善斑马鱼化 (ZnCl2) 暴露引起的认知障碍方面的疗效.
- 评估ZnCl2及其后的益生菌治疗对斑马鱼行为和认知功能的影响.
主要方法:
- 斑马鱼被暴露在0.5 mg/L和1.0 mg/L的ZnCl2中,持续96小时.
- 暴露后,斑马鱼接受了为期7天的益生菌治疗 (Bifidobacterium longum,B. animalis lactis,Lactobacillus rhamnosus).
- 行为评估包括社交能力,使用EthoVision XT 16软件进行短期和长期记忆测试.
主要成果:
- 暴露于ZnCl2会影响认知功能和社会行为 (0.5 mg/L).
- 益生菌治疗没有显著地扭转由ZnCl2.2引起的认知缺陷.
- 在1.0mg/L的ZnCl2暴露后给予益生菌,表明斑马鱼具有抗焦虑作用.
结论:
- 益生菌可能对化毒性产生部分神经行为益处,主要是通过减少类似焦虑的行为.
- 在这种斑马鱼模型中,益生菌没有显著改善ZnCl2诱导的认知障碍.
- 需要进一步研究,以了解涉及重金属神经毒性和益生菌干预的肠-大脑轴机制.
相关概念视频
01:23Drugs Affecting GI Tract Motility: Serotonin Receptor Agonists
Serotonin, a crucial neurotransmitter synthesized by enterochromaffin cells, plays a cardinal role in regulating gastrointestinal (GI) motility. With over 90% of the body's total serotonin in the GI tract, its influence on digestive processes is profound. Serotonin is swiftly released upon various stimuli, such as food boluses or certain drugs, triggering intrinsic sensory neurons in the myenteric plexus and extrinsic vagal and spinal sensory neurons. This leads to the activation of the...
01:18Dysbiosis of the Gut Microbiota
The human gut microbiome includes a diverse array of microbial species, including beneficial commensals and opportunistic pathogens, which interact to support host health. These microbes contribute to essential functions such as nutrient metabolism, immune system modulation, and maintenance of intestinal barrier integrity. However, disruptions to this equilibrium—referred to as dysbiosis—can have widespread physiological consequences.Dysbiosis is often characterized by reduced microbial...
01:22Gut-Brain Axis
The gut–brain axis is a bidirectional communication system that connects the gastrointestinal tract and the brain. This interaction is mediated through multiple pathways, including the vagus nerve, hormonal signals, immune responses, and chemical messengers produced by gut microbes.Microbial Contributions to Brain FunctionGut microbiota contributes significantly to brain function by producing neuroactive compounds. These include neuroactive compounds that influence neurotransmitters such as...
01:21Microbiota Modulation by Antibiotics
Antibiotics have revolutionized modern medicine by saving countless lives from bacterial infections. However, their widespread use has inadvertently harmed the delicate balance of the human gut microbiota. The gut microbiota, a complex community of bacteria, archaea, viruses, and fungi, plays a vital role in regulating metabolism, immune responses, and maintaining intestinal health. Antibiotics, especially broad-spectrum types, disrupt this ecosystem by eradicating both harmful and beneficial...
01:29Hepatic Encephalopathy
DefinitionHepatic encephalopathy is a reversible neurologic syndrome that results from advanced liver dysfunction or portosystemic shunting. It leads to disturbances in cognition, behavior, and motor function due to the brain’s exposure to gut-derived toxins that the liver fails to detoxify.EtiologyThis condition develops either in the setting of acute fulminant hepatitis or progressively during chronic liver disease, such as cirrhosis and portal hypertension. Portosystemic shunting—including...