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工程大肠杆菌为高效生产L-Tryptophan的工程

Song Yang1, Shengyu Zhou1, Quanfeng Liang2

  • 1The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

Applied biochemistry and biotechnology
|March 26, 2025
PubMed
概括

研究人员通过优化新陈代谢途径和减少能源消耗,对大肠杆菌进行了改造,以提高L-三胺的产生. 这导致了一种高产品种,能够生产34.1g/L的L-三.

关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.结合策略 结合策略L-三甲是一种L-三甲.代谢工程是代谢工程.

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...