来自乳酸细菌Pediococcus acidilactici的曼纳酶:生产优化,净化和在果汁澄清中的应用
Jingjun Shi1, Xi Chen1, Ruoxi Yang1
1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, Heilongjiang, People's Republic of China.
Applied biochemistry and biotechnology
|March 26, 2025
概括
Pediococcus acidilactici HDM2产生增强的曼南酶,这是食品应用的安全酶. 优化使酶产量增加了39.3%,显示了水果汁澄清的前景.
科学领域:
- 微生物学 微生物学
- 酶学 是一种酶学.
- 生物技术是生物技术.
背景情况:
- Pediococcus acidilactici是一种安全的乳酸细菌物种,被认为是微生物曼南酶的宝贵来源.
- 曼纳酶酶对于各种工业应用,包括食品加工至关重要.
研究的目的:
- 为了隔离和识别一种新的Pediococcus acidilactici菌株 (HDM2),用于曼南酶的生产.
- 使用响应表面方法 (RSM) 来优化曼南酶的产生.
- 为了评估水果果汁澄清中的纯化曼南酶的疗效.
主要方法:
- 通过形态,生理,生化和分子 (16S rDNA,rpoA) 分析进行菌株鉴定.
- 两个步骤的RSM用于确定mannanase生产的最佳条件 (葡萄糖度,初始pH).
- 酶净化和表征 (分子量测定).
- 在果汁澄清中的应用和与商业mannanase的比较.
主要成果:
- 我们成功地分离和识别了Pediococcus acidilactici HDM2.
- 优化条件 (17.5 g/L 葡萄糖,pH 4.6) 导致曼南酶活性增加39.3% (97.9 U/mL).
- 扩大的清除区域 (2.7至4.5厘米) 证实了增强的酶生产.
- 与商业酶相比,在八种果汁中的六种中,纯化mannanase (30 KDa) 显示出更高的澄清效率.
结论:
- 在Pediococcus acidilactici HDM2.2.中,RSM有效优化了曼南酶的产生.
- 增强的曼南酶显示出工业应用的巨大潜力,特别是在食品层面的工艺中,如果汁澄清.
- P. acidilactici HDM2 是生物技术用途的安全高效曼南酶的有希望的来源.
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