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相关概念视频

Conserved Binding Sites01:49

Conserved Binding Sites

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

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Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
13.9K
The Nucleosome01:19

The Nucleosome

1.2K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
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Protein Folding01:22

Protein Folding

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Overview
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Lipids as Anchors01:32

Lipids as Anchors

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In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
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相关实验视频

Updated: May 23, 2025

Analyzing and Building Nucleic Acid Structures with 3DNA
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Analyzing and Building Nucleic Acid Structures with 3DNA

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DNA-蛋白质结合是由短元素主导的.

Hong Chen1, Yongping Xu1, Hao Ge2

  • 1State Key Laboratory of Gene Function and Modulation Research, School of Life Sciences, and Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, 100871, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|March 26, 2025
PubMed
概括

这项研究确定了对DNA结合和基因调节至关重要的转录因子 (TF) 的定元素 (AEs). 新的AEEscape算法揭示了AE密度围绕结合部位如何影响基因表达.

关键词:
固定元件 (AE) 是一个固定元件.固定元件密度 (AED) 是指固定元件的密度.转录因子和DNA相互作用转录因子结合部位 (TFBS)

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Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
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Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

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Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
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Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 基因表达调节是复杂的,涉及到与DNA结合的转录因子 (TFs).
  • 了解TF-DNA相互作用是解读基因调节的关键.
  • 以前的方法测量了TF绑定,但缺乏详细的能源景观洞察力.

研究的目的:

  • 为了识别关键的DNA序列,或"定元素" (AE),控制TF结合亲和力.
  • 开发一种新的算法,AEEscape,用于精确建模TF绑定能量景观.
  • 为了研究AE密度和基因调控之间的关系.

主要方法:

  • 利用KaScape方法测量热力学平衡时的TF群体.
  • 在BEESEM方法的基础上开发了AEEscape算法.
  • 将实验数据与基因组数据集成,以分析TF结合位点 (TFBS).

主要成果:

  • 在WRKY和PU.1 TF中确定了3-4个基本对"定元件" (AE),对于TF结合亲缘关系至关重要.
  • AEEscape算法精确地模拟了特定位置的k-mer绑定能量格局.
  • 在TFBS周围发现了一个"能量漏斗",与AE密度 (AED) 直接相关.

结论:

  • 在TF-TFBS相互作用和基因调节中,AE发挥着主导作用.
  • AE密度 (AED) 是影响基因表达的关键因素.
  • 这些发现为通过TF结合位点工程来操纵基因表达提供了新的策略.