对山羊中Mycobacterium tuberculosis复杂感染的死后诊断技术的评估
Débora Jiménez-Martín1, Leonor Muñoz-Fernández2, Irene Agulló-Ros2
1Departamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba. Córdoba, Spain.
Veterinary microbiology
|March 26, 2025
概括
直接组织实时PCR是一种高度敏感的方法,用于验证死后山羊的Mycobacterium结核复合体 (MTBC) 感染. 这种技术为动物结核病控制计划提供了传统培养方法的可靠替代方案.
科学领域:
- 兽医微生物学 兽医微生物学
- 传染性疾病 传染性疾病
- 动物健康 动物健康
背景情况:
- 羊容易感染Mycobacterium结核复合体 (MTBC),影响动物结核病 (TB) 流行病学.
- 传统培养用于MTBC确认是缓慢的,敏感性低,需要严格的生物安全性.
- 替代性死后诊断技术对于有效的结核病控制和根除至关重要.
研究的目的:
- 为了比较各种死后诊断技术在结核素皮肤试验 (TST) 阳性山羊中MTBC确认的疗效.
- 评估直接组织实时聚合酶连锁反应 (RT-PCR) 与培养,总体/组织病变观察和Ziehl-Neelsen染色.
主要方法:
- 从205只TST阳性山羊收集了肺和淋巴结样本.
- 进行了直接的组织RT-PCR,MTBC培养,针对结核病样病变 (TBL) 的粗体和组织病理学检查,以及Ziehl-Neelsen染色.
- 利用 spoligotyping 来表征来自培养的MTBC分离物.
主要成果:
- 直接组织RT-PCR产生了最高的确认率 (86.3%),其次是组织病理学 (49.3%),严重病变 (47.8%),培养 (42.4%) 和Ziehl-Neelsen染色 (8.5%).
- 在体型分析中,发现了Mycobacterium caprae菌株SB0157 (86.0%) 和SB1081 (15.1%).
- 组织病理学揭示了肺和淋巴结样本中主要的瘤病变.
结论:
- 直接组织RT-PCR是一种敏感且可靠的尸检技术,用于确认山羊的MTBC感染.
- 对于山羊结核病控制计划来说,RT-PCR是有价值的,即使在没有毛病或组织病理病变的山羊中也是如此.
- 这种方法支持有效的动物结核根除策略.
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