由FOXM1转录调节的APOC1促进M2巨细胞两极分化和子宫癌进展
Qing Chai1, Yan Qi1, Xiaoyan Nie1
1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi 710077, China.
Mutation research
|March 26, 2025
概括
FOXM1/APOC1轴驱动子宫癌 (CC) 的进展和M2巨细胞的两极分化. 针对这种途径为CC治疗提供了新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
背景情况:
- 宫癌 (CC) 是女性普遍存在的恶性瘤.
- M2巨细胞有助于瘤生长,转移和免疫抑制.
- 脂蛋白C1 (APOC1) 是CC中确定的瘤基因,但其在CC进展和M2巨细胞调节中的精确作用和机制需要进一步研究.
研究的目的:
- 阐明APOC1在宫癌 (CC) 进展中的作用和机制.
- 为了研究APOC1对M2巨细胞极化的影响.
- 在CC中探索APOC1和叉盒M1 (FOXM1) 之间的监管关系.
主要方法:
- 在体外测试 (CCK-8,殖民地形成,伤口愈合,transwell) 和体内小鼠模型评估了APOC1对CC细胞表型的影响.
- 定量逆转录聚合酶连锁反应 (qRT-PCR) 和流细胞计评估了M2巨细胞的两极化.
- 染色体免疫沉 (ChIP) 和 luciferase 记者测定确定了 APOC1 和 FOXM1.1 之间的相互作用.
主要成果:
- 在CC组织和细胞中,APOC1和FOXM1的表达升高.
- 击败APOC1或FOXM1抑制了CC细胞的增殖,迁移,入侵和上皮细胞-介质细胞过渡 (EMT),并减弱了M2巨细胞的两极分化.
- 在体内,FOXM1转录激活APOC1和APOC1过度表达抵消了FOXM1的淘汰效应;APOC1淘汰减少了瘤生长和M2极化.
结论:
- FOXM1/APOC1轴促进子宫癌 (CC) 的进展.
- 这个轴在调节M2巨细胞极化方面发挥着至关重要的作用.
- FOXM1/APOC1通路为CC治疗提供了一个有前途的治疗标.
相关概念视频
02:23Master Transcription Regulators
6.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
02:23Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
01:06Induced Pluripotent Stem Cells
3.9K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
3.9K
03:03mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
01:46Epigenetic Regulation
30.8K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
30.8K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K