在牛植入前胚胎发育过程中追踪 lncRNA介导的竞争性内源性RNA网络的动态变化
Yuan Fan1, Krishna Chaitanya Pavani2, Osvaldo Bogado Pascottini3
1Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Journal of dairy science
|March 26, 2025
概括
长非编码RNAs (lncRNAs) 通过海绵式微RNAs (miRNAs) 调节牛胚胎的发育. 这些相互作用对于胚胎基因组激活周围的基因表达和胚胎细胞形成至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在基因表达中的调节作用.
- 在胚胎早期发育过程中,lncRNAs引发microRNAs (miRNAs) 的机制已经确立,但尚未得到充分研究.
- 了解lncRNA功能对于改善体外胚胎生产至关重要.
研究的目的:
- 为了研究 lncRNAs 在体外生产的牛前植入胚胎中的作用.
- 在牛早期发育过程中阐明lncRNA-miRNA-mRNA调节网络.
- 确定影响胚胎细胞形成的关键 lncRNA 中介途径.
主要方法:
- 重新分析现有的RNA测序和小RNA测序数据.
- 针对mRNA和lncRNA模块的权重基因共同表达网络分析 (WGCNA).
- 生物信息预测和构建lncRNA-miRNA-mRNA网络.
主要成果:
- 在胚胎基因组激活 (EGA) 过程中,母亲的lncRNAs比mRNAs更早地降解.
- 在多镇压复杂路径和胚胎囊形成之间发现了显著的负相关性 (R2 = -0.98).
- lncRNAs优先结合miRNAs,形成调节轴,影响mRNA降解和EGA周围的生物发生,相互作用加强后EGA.
结论:
- 在牛的植入前发育过程中,lncRNAs在调节基因表达方面发挥着重要作用.
- lncRNA-miRNA-mRNA网络对于发育过渡至关重要,特别是在EGA周围.
- 这些发现为管理早期牛胚胎发生的分子机制提供了新的见解.
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