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Updated: May 20, 2025
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Imaging of Extracellular Vesicles by Atomic Force Microscopy
Published on: September 11, 2019
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通过高速原子力显微镜 (HS-AFM) 视频摄影对小型细胞外囊泡进行纳米图谱分析
Muhammad Isman Sandira1,2, Keesiang Lim1, Takeshi Yoshida1,3
1WPI-Nano Life Science Institute, Kanazawa University, Kanazawa, Ishikawa, Japan.
Journal of extracellular vesicles
|March 26, 2025
概括
高速原子力显微镜 (HS-AFM) 揭示了小细胞外囊泡 (sEV) 内不同的亚群. 这项技术将CD63-CD81丰富的sEV与CD63-CD81贫乏的sEV区分开来,有助于发现生物标志物.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 纳米技术纳米技术
背景情况:
- 小型细胞外囊泡 (sEVs) 对于细胞间通信至关重要,并作为潜在的生物标志物.
- 目前的净化方法产生异质的sEV种群,限制了详细分析.
- 了解sEV异质性对于准确的生物标志物解释和治疗应用至关重要.
研究的目的:
- 在单胞分辨率下研究小细胞外囊泡 (sEVs) 的细胞内起源和异质性.
- 探索使用外体体标记器的高速原子力显微镜 (HS-AFM) 用于sEV表征的可行性.
- 根据表面标记物表达和物理性质来区分不同的sEV亚群.
主要方法:
- 利用高速原子力显微镜 (HS-AFM) 在生理条件下对HEK293T衍生的sEV进行成像.
- 使用外体标记抗体 (IgGCD63和IgGCD81) 来探测表面蛋白质定位.
- 分析了单个SEVs上的纳米拓学,结构动力学和标记器同定位.
主要成果:
- HS-AFM揭示了较大的 (直径>100 nm) 和较小的 (直径≤100 nm) sEVs之间明显的纳米拓学特征和高度波动.
- 外体标记抗体 (IgGCD63,IgGCD81) 显示出与较小的sEVs (sEVd ≤100 nm) 的主导同局部.
- 在异质混合物中证明了CD63-CD81丰富和CD63-CD81贫乏的sEV亚群的存在.
结论:
- 使用HS-AFM在sEV上的表面外体标记物的纳米显微镜分析是表征不同的sEV亚群的可行方法.
- 这种方法使异质sEV混合物的分化成为可能,为更精确的生物标志物发现铺平了道路.
- 这些发现突显了HS-AFM在促进对sEV异质性和功能的理解方面的潜力.
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