相关实验视频

Updated: Apr 11, 2026

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Published on: May 2, 2018

野生来源的小鼠中的自然遗传变异控制了宿主在内毒性冲击期间的生存和转录反应

Bristy Sabikunnahar¹, Julia P Snyder¹, Princess D Rodriguez²

¹Department of Biomedical and Health Sciences, University of Vermont, Burlington, VT, United States.

|March 26, 2025

概括

遗传变异在内毒性病期间显著影响生存和免疫反应. 野生来源的小鼠对脂聚糖诱导的冲击具有抗性,突出显示了潜在的败血症疗法的自然多样性,以调节潜在的败血症疗法的炎症途径.

科学领域:

免疫学免疫学免疫学
遗传学是一个遗传学.
系统生物学系统生物学

背景情况:

天生的免疫细胞对微生物信号发起炎症反应,对宿主防御至关重要,但如果失调,可能有害,如败血症休克.
目前的研究经常在小鼠模型中使用有限的遗传多样性,阻碍了对免疫反应的自然变异的充分理解.
内毒素,败血症的一个模型,涉及对细菌脂聚糖 (LPS) 的反应.

研究的目的:

调查自然遗传变异在宿主存活和免疫反应在内毒性病期间的作用.
为了比较基因分歧的小鼠菌株 (C57BL/6和PWD/PhJ) 之间的内毒素反应.
为了确定参与内毒性病的炎症途径的遗传调节者.

主要方法:

对C57BL/6 (B6) 和PWD/PhJ (PWD) 小鼠体内和体内内内毒素模型的比较.
脂聚糖 (LPS) 刺激骨髓衍生的树突细胞 (BMDCs),然后进行RNA测序.
在B6背景上分析一个具有PWD衍生的染色体11段的亚消费性菌株,以剖析cis-和trans-regulation.

主要成果:

与B6小鼠相比,PWD小鼠对LPS诱导的内毒性休克表现出明显的抵抗力.
来自B6和PWD小鼠的BMDC显示出明显的转录重编程:B6倾向于促炎途径,而PWD则参与了调节/发育途径.
在PWD衍生的染色体11段编码的候选转作用调节剂 (Irf1,Ncor1,Srebf1) 的识别.

结论:

自然遗传变异在内毒性病期间极大地影响宿主生存和转录反应.
不同的遗传背景导致不同的免疫细胞重编程策略.
研究结果表明,有潜力预测败血症风险,并根据基因构成开发个性化的治疗干预措施.

关键词:

动物 - 动物细胞 - - 状细胞疾病-内毒性休克疾病-内毒性休克分子-脂多多糖糖化物过程 - 基因调节

更多相关视频

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection

Published on: February 28, 2019

Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure

Operating and Biocontainment Procedures of a Facility for Laboratory Mice with a Natural Microbiome: Immunophenotyping Procedure

Published on: December 13, 2024

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Transduction

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...