探索FUT2基因中有害的非同义SNP,以及对诺罗病毒易感性和肠道微生物群组成的影响
Muhammad Waleed Iqbal1, Muneer Ahmad2, Muhammad Shahab1
1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Scientific reports
|March 27, 2025
概括
计算分析发现了两个Fucosyltransferase 2 (FUT2) 基因突变,G146S和V196G,可能增加诺罗病毒易感性并改变肠道微生物群. 这些nsSNP显示稳定结合于诺罗病毒VP1蛋白.
科学领域:
- 遗传学和分子生物学
- 病毒学 病毒学
- 计算生物学 计算生物学
背景情况:
- 基转移酶2 (FUT2) 基因影响肠道微生物群和诺病毒易感性.
- 非同义单核酸多态 (nsSNP) 可以破坏正常的FUT2功能.
研究的目的:
- 通过计算来研究nsSNP对FUT2基因的影响.
- 探索FUT2 nsSNPs,肠道微生物群和诺罗病毒易感性之间的潜在联系.
主要方法:
- 用九个生物信息学工具进行nsSNP查.
- 评估nSNP对FUT2.2的结构和功能影响.
- 执行了FUT2 nsSNP与诺罗病毒VP1蛋白的分子对接和动态模拟.
主要成果:
- 确定了两个nsSNP,G146S (rs200543547) 和V196G (rs367923363),可能具有有害的作用.
- 与野生类型相比,突变的nsSNP表现出较高的稳定性和较低的与诺罗病毒VP1的结合能.
- 分子动力学模拟证实了突变nsSNP的稳定性增加.
结论:
- 已识别的FUT2 nsSNP (G146S和V196G) 可能有助于诺病毒易感性.
- 计算策略对于分析nsSNP效应和预测疾病关联是有价值的.
- 建议进行进一步的实验验证,以确认这些发现.
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