相关实验视频
Updated: May 20, 2025
11:28
Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
17.9K
1,25(OH) 2D3对Dickkopf-1甲基化在结直肠癌中的作用
Hongyan Sun1, Liehao Yang2, Nan Li3
1Department of Biobank, China-Japan Union Hospital of Jilin University, Changchun, China.
Clinical epigenetics
|March 27, 2025
概括
维生素D (1,25(OH) 2D3) 通过降低DNA甲基化,通过降低结直肠癌中Dickkopf-1 (DKK1) 表达的调节. 这表明DKK1作为瘤抑制剂,提供潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 维生素D在结直肠癌 (CRC) 中起着保护作用.
- 维生素D会影响各种瘤中的DNA甲基化.
- Dickkopf-1 (DKK1) 抑制了Wnt/β-catenin通路;1,25(OH) 2D3可以诱导CRC中的DKK1表达.
研究的目的:
- 调查1,25(OH) 2D3是否通过结直肠癌中的DNA甲基化影响DKK1的表达.
- 分析血清DKK1,25(OH) D水平与CRC患者的临床病理特征之间的关联.
主要方法:
- 用ELISA和LC-MS/MS测量的血清DKK1和25(OH) D水平.
- DKK1的甲基化状况使用硫胺片测序 (BSAS) 和甲基化特异性PCR进行分析.
- 1,25(OH) 2D3对DKK1甲基化和转录的作用,通过热测序和双化酶记者分析进行了研究.
主要成果:
- 血清DKK1降低与神经透和25(OH) D状态相关于CRC患者.
- 在瘤组织中观察到DKK1过甲基化,与减少DKK1表达相关.
- 1,25(OH) 2D3处理通过去甲基化促进体和5'UTR区域诱导了DKK1的表达.
结论:
- 在结直肠癌中,DKK1作为瘤抑制剂起作用.
- 1,25(OH) 2D3通过其促进物和5'UTR的脱甲基化来调节DKK1的表达.
- 这些发现突出了维生素D在CRC中的作用的新型表观遗传机制.
关键词:
1,25 (((OH) 2D3 3D3 3D3 3D3 3D3 3D3 3D3 3D3 3D4 3D5 3D6 3D7 3D8 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9 3D9结肠直肠癌是一种癌症.DKK1 DKK1 的意思通过DNA甲基化.更多相关视频
相关概念视频
01:17Hormones and Bone Tissue
2.5K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.5K
03:24Common Ion Effect
40.8K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Châtelier’s principle. Consider the dissolution of silver iodide:
40.8K
04:01Factors Affecting Solubility
32.9K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide:
32.9K
01:21Skeleton and Calcium Homeostasis
4.3K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
4.3K
01:25Role of Vitamins in Maintaining Bone Health
3.1K
The growth and maintenance of bone are regulated by a combination of nutritional factors, including vitamins, such as vitamin A, B12, C, D, and K.
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...
3.1K
01:18Functions of Thyroid Hormones
2.4K
The thyroid hormone (TH) plays a pivotal role in the intricate orchestration of physiological processes, exerting profound effects on development, metabolism, and homeostasis throughout different life stages.
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
TH is indispensable for the normal development and maturation of the skeletal, muscular, and nervous systems during fetal and childhood growth. It facilitates bone mineral turnover and regulates protein synthesis in developing tissues, contributing significantly to overall growth and...
2.4K