Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

4.0K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
4.0K
Amyloid Fibrils03:03

Amyloid Fibrils

9.1K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
9.1K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.0K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.0K
Destabilization of Microtubules01:45

Destabilization of Microtubules

2.5K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Can amoxicillin be detected in the aquatic environment? A critical assessment.

Environment international·2026
Same author

MIF-Induced CD74+ Microglia and Macrophages Promote Progression of Brain Metastasis and Are Clinically Relevant across Central Nervous System Disorders.

Cancer research·2026
Same author

Sensory deficiencies correlate with tau protein and dementia.

Frontiers in neuroscience·2026
Same author

Neuronal alkaline phosphatase promotes the spread of Tau-induced pathology, and its blockade prevents neurodegeneration and memory loss.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2026
Same author

Performance comparison of microalgae-based and activated sludge with membrane filtration (AS-MBR) for emerging contaminant removal and wastewater reuse.

Journal of hazardous materials·2025
Same author

Structure and function of Full-length Tau.

PloS one·2025

相关实验视频

Updated: May 20, 2025

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
09:22

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Published on: January 2, 2015

18.3K

改变形状的W-Tau可以抑制tau的聚合,并分解配对的螺旋丝.

Indalo Domene-Serrano1,2, Raquel Cuadros1, Vega García-Escudero1,3

  • 1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Madrid 28049, Spain.

Biochemistry
|March 27, 2025
PubMed
概括

一种新型的W-Tau抑制了tau的聚合并破坏了现有的tau丝,为阿尔茨海默病等神经退行性tau病症提供了潜在的治疗益处.

更多相关视频

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
09:49

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Published on: November 20, 2018

18.4K
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
07:18

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

Published on: November 9, 2018

8.3K

相关实验视频

Last Updated: May 20, 2025

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
09:22

In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein

Published on: January 2, 2015

18.3K
In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
09:49

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

Published on: November 20, 2018

18.4K
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
07:18

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

Published on: November 9, 2018

8.3K

科学领域:

  • 神经科学是一个神经科学.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 病是一种神经退行性疾病,与异常的蛋白聚合有关.
  • 不同的tau拼接异型有助于形成这些有毒聚合物.

研究的目的:

  • 为了研究W-Tau的作用,从一个新的tau异型 (W-Tau) 衍生,在tau丝聚合和脱聚合.
  • 评估W-Tau在病的治疗潜力.

主要方法:

  • 在使用AlphaFold3 (AF3) 的化分析中预测W-Tau相互作用.
  • 在体外实验中证实W-Tau对tau聚合的影响.
  • 使用FRET细胞生物传感器,在W-Tau和PHF种子的存在下监测tau聚合.
  • 从阿尔茨海默病患者样本中分析PHF.

主要成果:

  • 预测W-Tau与tau单体相互作用,并证实它可以抑制tau聚合in vitro.
  • 该W-Tau有效地破坏了来自阿尔茨海默病大脑的预先形成的双螺旋丝 (PHF).
  • 当W-Tau与PHF种子共同转化时,tau聚合的减少被证明是W-Tau,由FRET生物传感器证实.
  • W-Tau可以通过将PHF分解成圆形结构来破坏PHF.

结论:

  • W-Tau在抑制和破坏聚合方面显示出显著的潜力,这表明它对病的治疗价值.
  • W-Tau异型及其衍生代表了未来针对病的神经保护干预的有希望的候选人.