在牛胚胎发育过程中对溶酸及其受体的影响的评估
Bo Yu1,2, Shuying Dai1,2, Lei Cheng3
1Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
International journal of molecular sciences
|March 27, 2025
概括
牛卵管液中的溶解酸 (LPA) 通过增加细胞数量和减少亡来增强胚胎发育. LPAR2被确定为参与LPA的关键受体.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- Lysophosphatidic 酸 (LPA) 是一种对胚胎发育至关重要的生物活性脂.
- 在各种物种中,LPA促进了体外生成 (IVP) 胚胎的发育,包括通过Hippo/YAP途径在牛中加速胚胎细胞形成.
- 在牛胚胎发育中LPA的确切作用和受体需要进一步阐明.
研究的目的:
- 调查牛卵管液中LPA的存在和对早期胚胎发育的影响.
- 为了确定潜在的LPA受体,参与调解LPA对牛胚胎的影响.
- 分析LPA对细胞亡和牛胚胎发育中的总细胞数的影响.
主要方法:
- 与酶相关的免疫吸收试验 (ELISA) 检测牛卵管液中的LPA.
- 终端脱核基转移酶 dUTP 末标记 (TUNEL) 试验和定量逆转录聚合酶链反应 (qRT-PCR) 来评估亡和基因表达.
- 分子对接,RNA-seq和qRT-PCR用于评估LPA受体 (LPAR) 表达和结合亲和力.
主要成果:
- 在牛卵管液中检测到LPA.
- 牛胚胎的LPA治疗导致了囊胚细胞数量的增加和亡的减少.
- GPR87和LPAR2表现出高的LPA亲和力,转录表达后16细胞阶段;LPAR2表达显著增加后LPA刺激在第6天芽细胞.
结论:
- LPA对牛胚胎发育有积极的影响,增加细胞数量并降低细胞亡.
- LPAR2是牛胚胎中LPA信号传递的潜在媒介,其表达由LPA上调.
- 这些发现有助于理解LPA在哺乳动物胚胎发育中的作用和信号通路.
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