优化和标准化来自植物的血管支架
Gianna Imeidopf1, Dara Khaimov1, Sashane John1,2
1Fred DeMatteis School of Engineering and Applied Science, Hofstra University, Hempstead, NY 11549, USA.
International journal of molecular sciences
|March 27, 2025
概括
优化的植物性支架对血管移植有很大的希望,在保持机械强度的同时,可以实现超过95%的DNA去除. 这项创新为合成材料提供了生物相容的替代品,改善了组织的整合,减少了移植失败.
科学领域:
- 生物材料工程 生物材料工程
- 组织工程是组织工程.
- 来自植物的细胞外基质.
背景情况:
- 由于血栓形成和不良整合,血管移植失败需要先进的生物材料.
- 合成移植和自身血管具有局限性,导致需要替代品.
- 植物衍生细胞外基质 (ECM) 提供了一个可扩展,生物相容的支架源.
研究的目的:
- 为了优化植物衍生ECM支架的脱细胞化协议.
- 评估加工参数对脚手架性能和生物性能的影响.
- 建立一个可复制的框架,用于开发植物衍生的血管移植.
主要方法:
- 使用二甲硫酸盐 (SDS) 和青素/特吉托尔治疗,去细胞化Leatherleaf viburnum叶.
- 使用漂白剂和Triton X-100的清除持续时间 (6-72小时) 的优化.
- 评估DNA去除 (>95%),机械性质 (拉力测试),结构完整性 (组织学) 和内皮细胞播种效率.
主要成果:
- 基于SDS的协议缩短了清除时间,实现了超过95%的DNA去除,并保留了机械性能.
- 较短的清除时间维持了ECM完整性,并提高了内皮细胞播种效率.
- 较大的叶子支持更高的内皮细胞密度,表明材料来源标准化的重要性.
结论:
- 优化的植物衍生ECM支架在机械上强大,在血管应用中具有生物兼容性.
- 这项研究为可再生的植物衍生移植开发提供了一个框架.
- 这些发现为改进血管组织工程解决方案铺平了道路.
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
01:17Autoregulation of Blood Flow
Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.
01:07Development of Blood Vessels
The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...