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一种模块化,经济高效,无输液组件,用于工程微容器的长期培养
Shashwat S Agarwal1, Jacob C Holter2,3, Travis H Jones1
1Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210, USA.
Micromachines
|March 27, 2025
概括
我们开发了一种低成本的模块化无 perfusion 组件 (PPA) 用于微流体细胞培养. 这种易于构建的系统使用易于获得的组件来实现可靠的长期细胞培养.
科学领域:
- 生物技术是生物技术.
- 微流体学 微流体学
- 细胞培养技术 细胞培养技术
背景情况:
- 连续 perfusion 对于微生理系统和微流体细胞培养来说至关重要.
- 现有的 perfusion 系统往往会带来运行挑战和高成本,限制了没有微系统工程专业知识的研究人员的可访问性.
研究的目的:
- 开发一个具有成本效益,易于实施和可靠的微流体 perfusion 系统.
- 为昂贵和复杂的现有 perfusion 技术提供替代方案.
主要方法:
- 使用商用注射器和注射器过器构建一个模块化无 perfusion 组件 (PPA).
- 液压电阻的理论计算和过器电阻的实验性表征.
- 在工程内皮质化3D微容器的长期培养中评估PPA性能.
主要成果:
- 该PPA是由低成本 (1-2美元/组装),可互换和无菌包装的组件构建的.
- 该系统通过结合高阻力过器,使微流体在长时间 (几个小时到几天) 的缓慢和持续流动.
- 使用PPA,在几周内实现了工程内皮质化3D微容器的可靠长期培养.
结论:
- 这种新型无 perfusion 组件 (PPA) 为微流体细胞培养提供了强大,简单和实惠的解决方案.
- 该PPA与现有的微流体装置兼容,并使用易于获得的实验室用品促进持续的细胞培养.
关键词:
液压电阻 液压电阻 液压电阻 液压电阻模块化的微流体学perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion perfusion 这是一个非常重要的方法,可以让你的身体和你的身体都变得更生动的,因为你的身体和你的身体都变得更生动的,因为你的身体和你的身体都变得更生动的论文 论文 论文 论文
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