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在THP-1巨中甜乳酪衍生的免疫调节性质

Eleni Dalaka¹, Georgios C Stefos¹, Ioannis Politis¹

¹Laboratory of Animal Breeding and Husbandry, Department of Animal Science, Agricultural University of Athens, 11855 Athens, Greece.

Molecules (Basel, Switzerland)

|March 27, 2025

概括

甜乳清 (SW) 分数调节巨细胞中的免疫基因表达,可能预防与炎症相关的疾病. 绵羊/山羊SW显示有前途的抗炎作用,表明其在功能性食品中的使用.

科学领域:

免疫学免疫学免疫学
营养科学营养科学
生物化学生物化学

背景情况:

甜乳清 (SW) 是一种乳制品副产品,具有潜在的免疫调节特性.
与炎症相关的疾病是一个重大的健康问题.
了解SW对免疫反应的影响对于其应用至关重要.

研究的目的:

研究SW分量的免疫调节作用对巨细胞中与炎症相关的基因表达.
为了比较来自不同动物乳源 (牛,山羊,绵羊,绵羊/山羊混合物) 的SW的影响.
在基底和脂聚糖 (LPS) 刺激条件下评估SW的影响.

主要方法:

用SW-D-P3 (分数<3kDa) 治疗THP-1衍生的巨细胞.
使用定量PCR来评估关键炎症相关基因的表达.
实验与没有LPS刺激进行.

主要成果:

在非LPS刺激的巨细胞中,SW-D-P3减弱的TLR2和ICAM1表达,不论牛奶来源如何.
根据SW来源观察到对TLR4,IL1B,IL6,RELA和CXCL8的不同影响.
在LPS刺激的细胞中,SW-D-P3调节了CXCL8,IRF5和TNFRSF1A的表达,因源而异.
绵羊/山羊SW-D-P3治疗导致IL10的表达更高,这表明M2巨分化.

结论:

甜糖乳清部分对巨细胞表现出免疫调节作用,影响关键的炎症途径.
牛奶的来源显著影响SW的免疫调节特征.
SW,特别是绵羊/山羊来源,具有作为预防炎症相关疾病的功能性食品成分的潜力.

关键词:

信息报道信息报道奶酪乳酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶酪奶奶奶奶奶奶奶奶奶奶奶奶奶奶奶奶奶奶奶这是一种炎症炎症炎症炎症.qPCRR 是一个很好的方法.

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