直接3D质谱成像环境微生物的分析
Justyna Szulc1, Tomasz Grzyb1, Joanna Nizioł2
1Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, 90-530 Lodz, Poland.
Molecules (Basel, Switzerland)
|March 27, 2025
概括
这项研究介绍了激光除远程大气压光离子化/化学离子化质谱成像 (LARAPPI/CI-MSI 3D) 用于在3D中可视化微生物代谢物. 这种新方法有助于从环境微生物中发现有价值的生物分子.
科学领域:
- 代谢学 代谢学 代谢学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 评估培养基中微生物代谢物的空间分布至关重要,但具有挑战性.
- 固体介质中的代谢物分布的直接3D可视化一直是有限的.
研究的目的:
- 引入和验证新的LARAPPI/CI-MSI 3D技术,用于直接对微生物代谢物的3D质谱成像.
- 证明LARAPPI/CI-MSI 3D在可视化固体培养基中的代谢物分布和度方面的能力.
主要方法:
- 开发并应用了激光除远程大气压光离子化/化学离子化质谱成像技术 (LARAPPI/CI-MSI 3D).
- 通过超高性能液体染色学-超高分辨率质谱学 (UHPLC-UHRMS) 执行2D MSI和相关结果.
- 识别和量化代谢物包括氨基酸,有机酸,脂肪酸和糖.
主要成果:
- 在固体培养基中成功可视化了16种代谢物的3D分布和度.
- 鉴定了每种微生物的351-393种化学化合物,在人类新陈代谢数据库 (HMDB) 中识别了242-262.
- 证明了各种代谢物类的存在,如氨基酸,糖和脂肪酸.
结论:
- LARAPPI/CI-MSI 3D是一种强大的新工具,用于直接对微生物代谢物的3D质谱成像.
- 这种方法可以快速选环境微生物菌株的生物技术潜力.
- 促进从微生物来源发现新型,具有工业价值的生物分子.
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