微RNA在膀过活中的作用:关系和临床相关性
Kürşat Küçüker1, Hülya Aybek2, Hakan Akça3
1Department of Urology, Pamukkale University School of Medicine, Denizli 20070, Turkey.
Medicina (Kaunas, Lithuania)
|March 27, 2025
概括
微RNAs (miRNAs) 在诊断过度活性膀 (OAB) 和预测治疗反应方面表现出潜力. 在OAB患者和对照人群之间,特定的miRNA水平有所不同,其中一些表明诊断价值和治疗结果.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 过度活性膀 (OAB) 是一种具有复杂病理生理学的疾病.
- 微RNAs (miRNAs) 涉及到各种生物过程和疾病.
- 了解miRNAs在OAB中的作用可以提供新的诊断和治疗见解.
研究的目的:
- 为了研究特定的miRNAs和过度活跃的膀 (OAB) 之间的关系.
- 评估miRNAs对OAB的诊断潜力.
- 探索miRNAs对OAB患者治疗反应的预测价值.
主要方法:
- 来自60名OAB患者和60名健康对照者的血液样本使用PCR分析了15个miRNA.
- 用接收器操作特征 (ROC) 曲线来识别具有高诊断价值的miRNA.
- 在治疗前和治疗后使用了土耳其验证的OAB问卷,以评估临床改善及其与miRNA水平的相关性.
主要成果:
- 五个let-7家族的miRNAs (let-7a,let-7c,let-7e,let-7f,let-7g) 在OAB患者中显著更高 (p=0.0001).
- 其他一些miRNAs (miR-135b,miR-300,miR-372,miR-373,miR-381,miR-520a,miR-520d,miR-520e) 在对照组中显著高 (p=0.0001).
- let-7c (AUC=0.985) 和let-7c + miR-381组合 (AUC=1) 显示出高的诊断准确性. 在let-7f和miR-135b的变化与治疗后OAB患者的临床改善相关.
结论:
- 在过度活动的膀的病理生理学中,MiRNAs起着重要作用.
- 特定的miRNAs显示了OAB的诊断潜力.
- 微RNA分析可能有助于评估OAB治疗的有效性.
相关概念视频
01:22MicroRNAs
21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
01:23Urinary Bladder
4.7K
The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
4.7K
01:26The Micturition Reflex
4.1K
Urination, or micturition involves the coordination of the bladder's detrusor muscle and two sphincters to ensure controlled bladder emptying.
The process begins with bladder filling, where the bladder wall stretches as urine accumulates. This stretching activates the urine storage reflex, mediated by the sacral spinal segments and the pontine storage center. Efferent sympathetic impulses stimulate the detrusor muscle to relax and the internal urethral sphincter to contract, facilitating...
The process begins with bladder filling, where the bladder wall stretches as urine accumulates. This stretching activates the urine storage reflex, mediated by the sacral spinal segments and the pontine storage center. Efferent sympathetic impulses stimulate the detrusor muscle to relax and the internal urethral sphincter to contract, facilitating...
4.1K
01:26Urinary Tract Calculi II: Pathophysiology and Clinical Manifestations
860
Renal calculi, commonly termed kidney stones, are crystalline solid masses that form in the kidneys but can occur at any point within the urinary system, encompassing the kidneys, ureters, bladder, and urethra.The pathophysiology of renal stones involves several key factors: supersaturation of the urine with stone-forming constituents, changes in urine pH, a decrease in urine volume, and the presence of substances that promote or inhibit stone formation.Supersaturation of Urine: This is the...
860
01:28Microbiota of the Urogenital Tract
53
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
53