在Trichoderma reesei中DNA甲基化对细胞质生产和菌株退化的影响
Caroline Danner1, Thiago M Mello de Sousa1, Robert L Mach2
1Christian Doppler Laboratory for Optimized Expression of Carbohydrate-Active Enzymes, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorfer Str. 1a, 1060 Vienna, Austria.
Microorganisms
|March 27, 2025
概括
通过DNA甲基化的表观遗传调节会影响工业Trichoderma reesei (T. reesei) 菌株的细胞酶产生. 了解这种联系有助于减少菌株退化并改善酶制造.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 工业微生物学 工业微生物学
背景情况:
- 工业Trichoderma reesei (T. reesei) 菌株自发失去细胞质生产力,造成经济损失.
- 据怀疑这种退化是由表观遗传调节的,但具体机制尚不清楚.
- 对于T. reesei的表观遗传情景,特别是DNA甲基化,人们对其了解甚微.
研究的目的:
- 研究T. reesei.中的DNA甲基化与细胞酶产生之间的联系.
- 为了阐明DNA甲基化如何影响菌株退化.
- 探索减轻细胞酶生产损失的潜在策略.
主要方法:
- 使用氨酸化诱导的低甲基化.
- 全球DNA细胞因子甲基化试验量化5-甲基细胞因子 (5-mC) 水平.
- 删除实验针对假定的DNA甲基转移酶 (Dim2和Rid1).
主要成果:
- 低甲基化部分恢复了细胞酶的生产力,并减少了非生产性表型.
- 在T. reesei中,DNA甲基化水平在0.2%至1.3%之间.
- 非生产性菌株的甲基化程度高于生产性菌株;甲基化模式因碳源而异.
- 删除Dim2和Rid1最初减少了非生产子种群,但长期种植导致了生产力的完全丧失.
结论:
- DNA甲基化直接影响T. reesei细胞酶的生产力和菌株稳定性.
- 向DNA甲基化提供了一种潜在的策略来对抗菌株退化.
- 通过管理表观遗传因素,进一步的研究可以优化工业酶生产.
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