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细菌在软表面上的粘附:基质刚度和细菌生长阶段的双重作用

René Riedel¹, Garima Rani¹, Anupam Sengupta^1,2

¹Physics of Living Matter Group, Department of Physics and Materials Science, University of Luxembourg, 162 A, Avenue de la Faïencerie, 1511 Luxembourg, Luxembourg.

|March 27, 2025

概括

细菌对表面的粘附受基质刚性的影响. 这项研究量化了硬度如何影响细菌的附着,揭示了特定物种的特征和生物膜发育至关重要的多样性粘附表型.

科学领域:

生物物理学的生物物理.
微生物学微生物学
细胞力学细胞力学

背景情况:

基质的刚性和表面粘附性影响细菌殖民地形成.
之前的研究表明,硬度对细菌的附着有质性的影响,但缺乏定量数据.

研究的目的:

量化基板刚度和细菌细胞表面粘附之间的关系.
为了研究硬度调节粘附的特定物种性质.
探索随着时间的推移细菌粘附表型的多样性.

主要方法:

开发了一种使用原子力显微镜 (AFM) 的细胞水平力距离光谱 (FDS) 技术.
同时量化细胞表面粘附和基质刚度.
使用低点 (LMP) 亚加罗斯片的各种基质刚度,度从20kPa到120kPa.

主要成果:

*Chromatium okenii*的平均附着力随着基板刚度的增加而增加了一大数 (0.21±0.10 nN至2.42±1.16 nN).
被动聚钢 (PS) 微粒的粘附性没有变化,证实了 *C. okenii* 的生物起源.
*大肠杆菌表现出弱硬度依赖 (0.29±0.17nN到0.39±0.20nN),表明了特定物种的特征.
在 *C. okenii* 中观察到弱粘附和强粘附子群的共存,在成长阶段进行多样化.

结论:

细菌积极调节细胞表面粘附,以应对基质刚性,这取决于物种和生理阶段.
基质刚度是一个关键的生物物理参数,影响细菌的附着和殖民,以及水性等表面特性.
硬度依赖的粘附和表型多样性是细菌殖民和软基板上的生物膜形成的功能特征.

关键词:

航空飞行管理 (AFM)粘附性粘附性粘附性粘附性细菌细菌细菌是一种细菌.生物膜是一种生物膜.强力位移光谱学现型多样性现型多样性表面的硬度表面的硬度

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