在乳腺癌细胞系中评估CTCF在IL6基因转录中的调控作用
Angel Francisco Pacheco-Hernandez1, Itayesitl Rodriguez-Ramos1, Karla Vazquez-Santillan2
1Research Division, School of Medicine, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
Pharmaceuticals (Basel, Switzerland)
|March 27, 2025
概括
乳腺癌 (BrCa) 中高水平的插白素-6 (IL6) 与糟糕的结果相关. CCCTC-结合因子 (CTCF) 通常抑制IL6,但这种调节在攻击性的BrCa细胞中丢失.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 乳腺癌 (BrCa) 中的高IL6表达与不良的临床结果有关.
- CCCTC结合因子 (CTCF) 在调节BrCa.Ca中的基因表达方面发挥作用.
- 改变CTCF结合部位占用率可以去调节BrCa中的基因表达.
研究的目的:
- 研究CTCF在调节乳腺癌IL6表达中的作用.
- 为了确定CTCF是否有助于改变BrCa中的IL6水平.
- 探索BrCa.IL6中CTCF-IL6关系的临床影响.
主要方法:
- 在 BrCa 细胞系中进行 CTCF 功能增益和丧失测定.
- 路西法酶基因记者测试,位点定向突变发生和染色体免疫沉测试.
- 对TCGA和GEO数据库进行临床相关性分析.
主要成果:
- 观察到CTCF和IL6表达水平之间的反相关性.
- CTCF直接与IL6促进体相互作用,抑制其表达.
- 这种CTCF介导的调节在侵略性的BrCa细胞系 (MDA-MB-231,耐托莫西芬的MCF7) 中被破坏,而不依赖于CpG甲基化.
结论:
- 在乳腺癌中,CTCF负面调节IL6基因表达.
- 在高度瘤的BrCa细胞中发生了CTCF介导的IL6负调节的丧失.
- 这种失调可能导致与BrCa中的高IL6水平相关的不良结果.
相关概念视频
Master Transcription Regulators
6.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Regulation of Hematopoietic Stem Cells
3.1K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.1K
Cell Specific Gene Expression
13.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.3K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
Transcription Factors
75.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.5K


