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相关概念视频

ATP Energy Storage and Release01:31

ATP Energy Storage and Release

9.0K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
9.0K
Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

13.1K
In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
13.1K
ATP Yield01:31

ATP Yield

68.4K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
68.4K

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相关实验视频

Updated: May 20, 2025

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
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来自d-果糖的ATP再生的无细胞反应系统.

Franziska Kraußer1, Kenny Rabe1, Christopher M Topham2

  • 1Chair of Bioprocess Engineering, Institute of Natural Materials Technology, TU Dresden, Bergstraße 120, 01062 Dresden, Germany.

ACS synthetic biology
|March 27, 2025
PubMed
概括

这项研究介绍了使用d-果糖的无细胞腺三酸盐 (ATP) 再生系统. 优化的酶可以实现高ATP产量,使工业生物工艺更加可行.

关键词:
在ATP辅助因子的再生过程中,乙烯酸酸盐的合成方法在体外生物催化剂.分子建模分子建模光基托拉酶是一种光基托拉酶.半导体酶工程 半导体酶工程

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An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
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科学领域:

  • 生物技术是生物技术.
  • 酶工程是什么? 酶工程是什么?
  • 代谢工程是代谢工程.

背景情况:

  • 依赖亚丁三酸盐 (ATP) 的体外生物过程对于工业应用,如无细胞蛋白质合成至关重要.
  • 高昂的ATP成本是这些生物工艺广泛实施的一个主要限制.
  • 开发高效,经济的ATP再生系统对于推进这些技术至关重要.

研究的目的:

  • 开发和演示一种使用低成本基质的新型无细胞ATP再生系统.
  • 为提高ATP生产效率,设计了一种改进的光基托拉酶酶.
  • 为了优化整体酶级联以最大限度地提高ATP产量和生产力.

主要方法:

  • 设计了一个无细胞系统,利用从d-果糖和无机酸盐中产生的酸乙烯.
  • 采用半理性工程方法来优化Bifidobacterium adolescentis phosphoketolase (Bad.F6Pkt) 的使用.
  • 集成工程Bad.F6Pkt,乙酸激酶,甘激酶和l-rhamnose异构酶成为一个完整的ATP再生级联.

主要成果:

  • 改造的Bad.F6Pkt变种H548N显示d-果糖,d-红糖和糖类甲的活性显著增加.
  • 从d-果糖中证明了ATP再生,其固态度为每molC6基1molATP.
  • 整个系统的ATP产量为2.53mol ATP/mol d-果糖,生产率为7.2mM/h.

结论:

  • 开发的无细胞ATP再生系统有效地利用了低成本的d-果糖.
  • 基酶的酶工程对于提高ATP生产效率至关重要.
  • 该系统为工业ATP依赖生物工艺提供了具有成本效益的解决方案.