相关实验视频

Updated: May 20, 2025

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Published on: March 20, 2018

高分辨率的亚细胞结构的多模式成像,具有较低的数值孔径目标

Somaiyeh Khoubafarin¹, Peuli Nath¹, Saloni Malla²

¹Department of Physics and Astronomy, University of Toledo, Toledo, OH 43606, United States of America.

JPhys photonics

|March 27, 2025

概括

这项研究引入了低数值光圈 (NA). 使用微镜基板实现高分辨率亚细胞成像的客观镜头方法. 这种具有成本效益的技术增强了显微镜,使其更广泛的科学可访问性.

科学领域:

生物医学成像技术生物医学成像技术
显微镜技术显微镜技术
细胞生物学细胞生物学

背景情况:

高数值光圈 (N.A.) 的数值孔径目标对子细胞成像至关重要,但成本昂贵,复杂,视野有限.
目前的显微镜方法往往需要浸泡油,并且只能一次成像几个细胞.

研究的目的:

开发一种具有成本效益和可访问的方法,用于高分辨率的亚细胞结构的成像,使用低的NA. 目标,目标,目标.
为了证明同时捕捉散射,相位和光图像,具有有限分辨率的分辨率.

主要方法:

使用一个二维 (2D) 微镜片基板,靠近样品.
利用有效收集含有高频信息的 evanescent 波.
雇佣了一个低的NA. 目标 (0.25 N.A.) 的目标. 用于成像乳腺癌细胞 (BT-20).

主要成果:

实现了低射限度分辨率 (<400 nm) 的低N.A. 客观的,客观的,客观的
成功观察了乳腺癌细胞中的亚细胞结构和纳米粒子吸收.
证明了同时捕捉散射,相位和光图像.

结论:

微镜头辅助成像提供了一个易于实施的,具有成本效益的解决方案,以提高低NA的显微镜中的分辨率. 目标,目标,目标.
这项技术可以带来负担得起的,便携式的多模式成像系统.
对民主化高质量显微镜的广泛影响,特别是在资源有限的环境中.

关键词:

视野视野视野视野视野视野微镜的基板是微镜的基板.解决方案的解决方案解决方案的解决方案.

更多相关视频

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Published on: July 7, 2017

相关概念视频

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...