脂聚糖剂对断奶小猪体损伤,抗氧化能力和免疫功能的影响
Lingna Bai1, Yijie Jiang1, Xi Li2
1College of Animal Science and Technology, Jiangxi Agricultural University, 330045 Nanchang, Jiangxi, China.
Journal of veterinary research
|March 27, 2025
概括
在小猪中,由脂聚糖 (LPS) 诱导的断奶压力最初会激活胸腺免疫系统. 接下来是免疫抑制和缩,到第13天免疫功能逐渐恢复.
科学领域:
- 动物科学动物科学
- 免疫学 免疫学 免疫学
- 生物化学 生化学
背景情况:
- 断奶小猪由于环境和营养变化而经历压力.
- 压力会影响免疫系统,特别是胸腺,影响整体健康和生长.
- 脂聚糖 (LPS) 是一种强大的免疫刺激剂,用于模拟应激反应.
研究的目的:
- 调查LPS诱导的压力对小猪胸腺抗氧化指数和免疫炎症因子的影响.
- 了解在压力期间与免疫和抗氧化途径相关的胸膜基因表达的动态变化.
主要方法:
- 48只小猪被分为LPS治疗组和对照组.
- 每天服用LPS (100μg/kg) 或盐水,持续13天.
- 用RT-qPCR分析了胸膜组织的抗氧化酶活性和关键免疫基因的mRNA表达.
主要成果:
- 在胸腺中,LPS诱导了抗氧化酶 (甲酸酶,超氧化失调酶,谷氨) 的显著变化.
- 早期 (第1天) 观察到托尔类受体4 (TLR4) 和炎症性细胞因子 (IL-6,IL-2) 的上调.
- 实验中期 (5日) 显示了多种免疫通路 (TLR,JAK/STAT,Keap1/Nrf2) 和细胞因子的下调,表明免疫抑制.
- 后期阶段 (13日) 显示TLR4,Keap1/Nrf2通路和炎症类细胞因子表达的复苏.
结论:
- 连续使用LPS最初会过度刺激小猪胸腺免疫系统.
- 接下来是一段时间的胸腺缩和免疫抑制.
- 小猪的免疫系统在暴露于压力的后期阶段表现出逐渐恢复的能力.
关键词:
抗氧化能力的抗氧化能力.这是一种炎症炎症炎症炎症.脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.小猪小猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪胸腺 (thymus) 是一个神经系统.更多相关视频
08:24Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
Published on: May 2, 2018
19.3K
05:52Author Spotlight: Induction of Experimental Endotoxemic Shock in Pigs for Studying Hemodynamic and Respiratory Failure
Published on: December 8, 2023
845
相关概念视频
01:19Overview of Carbohydrate Metabolism
Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
01:22Diabetic Neuropathy
DefinitionDiabetic neuropathy is nerve damage caused by long-standing diabetes mellitus. It results directly from prolonged high blood sugar levels.PathophysiologyThe pathophysiology of diabetic neuropathy involves both metabolic and vascular disturbances triggered by chronic hyperglycemia.Metabolic injury: Elevated glucose levels activate the polyol pathway within nerve cells, leading to the accumulation of sorbitol and fructose. This increases oxidative stress, disrupts normal nerve...