Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

3.8K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
3.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Acute effects of cadmium and zinc on crayfish (Procambarus clarkii): Insights from deep learning-based behavioral analysis and physiological assessments.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Simulation of reaction-diffusion equations with reaction-reaction analog circuits.

Biophysical journal·2026
Same author

Six Fundamental Behaviors of Immune-Pathogen Feedback Circuits.

IEEE transactions on computational biology and bioinformatics·2025
Same author

Gene syntaxes modulate gene expression and circuit behavior on plasmids.

Journal of biological engineering·2025
Same author

Multi-omics analysis and longitudinal study of reprogramming by dietary creatine to endogenous metabolism in largemouth bass (Micropterus salmoides).

Fish physiology and biochemistry·2024
Same author

A Compact and Power-Efficient Noise Generator for Stochastic Simulations.

IEEE transactions on circuits and systems. I, Regular papers : a publication of the IEEE Circuits and Systems Society·2024

相关实验视频

Updated: May 20, 2025

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

10.8K

MutaT7的增长合连续定向进化使得高效和自动化的酶工程成为可能.

Yijie Deng1, Kai Etheridge1, Xinping Ran2

  • 1Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA.

Applied and environmental microbiology
|March 27, 2025
PubMed
概括

我们开发了一种增长合的持续定向进化 (GCCDE) 方法,用于自动化酶工程. 这种方法将酶活性与细菌生长联系起来,使得提高酶变异的高通量选择能够有效地进行.

关键词:
在 MutaT7T7 中使用.持续的进化,不断的演变.酶工程是指酶工程的工程.增长相结合的方向进化演变.在活体中发生突变的突变发生

更多相关视频

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.4K
Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
07:26

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Published on: May 19, 2019

11.9K

相关实验视频

Last Updated: May 20, 2025

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

10.8K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.4K
Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
07:26

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Published on: May 19, 2019

11.9K

科学领域:

  • 生物技术是生物技术.
  • 酵素工程是什么意思 酵素工程
  • 蛋白质工程是指蛋白质工程.

背景情况:

  • 传统的定向进化是劳动密集型和低通量.
  • 酶工程需要更快的,自动化的方法来改善或新的特征.

研究的目的:

  • 开发一种自动化,高通量酶工程方法.
  • 在较低的温度下增强酶活性,同时保持稳定性.

主要方法:

  • 开发了与增长合的持续定向进化 (GCCDE).
  • 使用MutaT7系统进行体内突变发生.
  • 与大肠杆菌中的细菌生长相关的酶活性用于选择.
  • 采用连续培养用于自动化突变发生和选择.

主要成果:

  • 在较低的温度下增强β-galactosidase活性的工程 CelB 酶.
  • 每种培养实现了超过10^9个变种的选择.
  • 确定了负责提高酶性能的关键突变.
  • 在工程 CelB 变体中保持热稳定性.

结论:

  • GCCDE是一种广泛适用的,自动化和高效的酶工程方法.
  • 这种方法绕过了传统的代步骤,加速了酶优化.
  • 显示了在酶开发中的工业和研究应用的潜力.