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在Ganoderma lucidum中,GCN4-Swi6B模块调解了低诱导的细胞壁重塑

Lingyan Shi¹, Lingshuai Wang¹, Rui Liu¹

¹Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.

Applied and environmental microbiology

|March 27, 2025

概括

缺会增加Ganoderma lucidum的细胞壁厚度和多糖含量. 这通过GCN4-Swi6B信号通路发生,揭示了真菌适应环境压力的关键机制.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
环境微生物学环境微生物学

背景情况:

菌面临环境压力,如缺乏,需要细胞壁适应.
细胞壁对于真菌与环境的相互作用至关重要,但低对其厚度和分子调节的影响尚不清楚.

研究的目的:

为了研究低对Ganoderma lucidum细胞壁厚度和多糖含量的影响.
阐明分子机制,特别是GCN4-SWI6B途径在调节缺乏下细胞壁重塑中的作用.

主要方法:

在正常和低条件下对Ganoderma lucidum细胞壁厚度和多糖含量的比较分析.
在野生型,过度表达和淘汰菌株中分析SWI6B和GCN4的基因表达.
染色体免疫沉以评估GCN4与SWI6促进体的结合.

主要成果:

低气条件增加了Ganoderma lucidum的细胞壁厚度和多糖含量.
转录因子SWI6B的丰度在低气条件下增加,并与细胞壁厚度呈正相关.
GCN4直接与SWI6促进体结合,积极调节SWI6B的表达,随后影响细胞壁厚度.

结论:

GCN4-Swi6B信号模块是缺乏症下Ganoderma lucidum细胞壁重塑的关键调节器.
这一途径代表了一种适应性反应,使真菌能够应对低环境.
这些发现提高了对真菌环境应激反应和细胞壁适应性的理解.

关键词:

在 GCN4 中.这种植物叫做Ganoderma lucidum.在 Swi66 中使用.细胞壁的厚度细胞壁的厚度低含量的低含量的人.

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