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Updated: May 20, 2025

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

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高通量单分子显微镜具有可适应的空间分辨率,使用可交换的寡核酸标签

Klarinda de Zwaan1, Ran Huo1, Myron N F Hensgens2

  • 1Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 2629 HZ Delft, The Netherlands.

ACS nano
|March 27, 2025
PubMed
概括

这项研究引入了一个更快的生物成像工作流程,结合了超分辨率光学波动成像 (SOFI) 和DNA-PAINT显微镜. 这种方法使细胞结构成像加速30-300倍,提高了生物分子研究的吞吐量.

关键词:
这就是DNA-PAINT.闪的动力学 闪的动力学光波动成像技术 光波动成像高通量显微镜的显微镜.单分子局部化显微镜.超高分辨率成像成像技术

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Conducting Multiple Imaging Modes with One Fluorescence Microscope

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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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