刺激性突触中的后突触密度由聚类的,异质的纳米块组成
Rong Sun1,2, James P Allen2, Zhuqing Mao1,2
1Department of Cell and Developmental Biology, Center for Structural Biology, Vanderbilt Kennedy Center, Vanderbilt University, Nashville, TN, USA.
The Journal of cell biology
|March 27, 2025
概括
这项研究可视化了突触的纳米结构,揭示了后突触密度包括多样化的纳米块. 这种组织是突触可塑性和功能的关键.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 结构生物学 结构生物学
背景情况:
- 突触传输和可塑性依赖于突触内的蛋白质精确的纳米级组织.
- 了解突触组件的三维架构对于阐明神经功能至关重要.
研究的目的:
- 通过使用冷电子断层扫描 (cryo-ET) 直接可视化后突触组件的3D架构和超分子组织.
- 在纳米尺度上研究 postsynaptic密度 (PSD) 的组成和排列.
主要方法:
- 低温电子断层扫描 (cryo-ET) 用于从培养的神经元中成像突触体和突触.
- 亚断层图片的平均值被用来解决后突触受体样粒子的结构.
主要成果:
- 化ET揭示了后突触密度 (PSD) 由不同尺寸的异质,膜相关的纳米块形成.
- 在高分辨率 (24-26 Å) 确定了两种类型的后突触受体样颗粒 (A型和B型).
- 前突触释放点似乎更接近与受体粒子相关的纳米块,这表明功能组织.
结论:
- 该PSD由聚类的,异质的纳米块组成,有助于其动态性和突触强度的调制.
- 这样可以更全面地了解突触超结构和后突触部位的蛋白质组织.
相关概念视频
Chemical Synapses
8.6K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
8.6K
Excitatory and Inhibitory Effects of Neurotransmitters
9.6K
When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of...
9.6K
Postsynaptic Potential (PSP)
2.2K
Postsynaptic potential (PSP) refers to a change in the electrical potential of a neuron when neurotransmitters released by presynaptic neurons bind to postsynaptic receptors. This potential can either be excitatory, leading to depolarization and ultimately action potential generation, or inhibitory, leading to hyperpolarization and suppression of the postsynaptic neuron.
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
There are two types of receptors: ionotropic and metabotropic.
The ionotropic receptor is the membrane protein that has an...
2.2K
The Synapse
121.1K
Neurons communicate with one another by passing on their electrical signals to other neurons. A synapse is the location where two neurons meet to exchange signals. At the synapse, the neuron that sends the signal is called the presynaptic cell, while the neuron that receives the message is called the postsynaptic cell. Note that most neurons can be both presynaptic and postsynaptic, as they both transmit and receive information.
121.1K
Synaptic Signaling
74.6K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
74.6K
Overview of Synapses
2.1K
A synapse is a specialized structure where two neurons connect, allowing them to pass an electrical or chemical signal to another neuron. It is the point of communication between neurons. The term "synapse" is derived from the Greek word "synapsis," which means "conjunction." The entire process of neural communication revolves around the synapse. When activated, a neuron releases chemicals known as neurotransmitters into the synapse. These neurotransmitters cross the...
2.1K


