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Updated: Aug 13, 2026

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使用CRISPR/Cas9产生TP53淘汰诱导的多能干细胞

Haonan Zhou1, Yaonan Liu2, Qu Chen3

  • 1Center for Cancer Immunology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Shenzhen International Institute for Biomedical Research, Longhua District, Shenzhen, Guangdong 518116, China.

Stem cell research
|March 27, 2025
PubMed
概括

我们使用CRISPR/Cas9.9创建了一个TP53基因淘汰诱导的多能干细胞系 (SIIBRi001-A). 这种细胞系保留了多能性,可以用来研究癌症发育机制.

关键词:
这就是CRISPR/Cas9的作用.这就是TP53的特点.这就是 iPSC 的意义.

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...