转录因子MdNAC18.1调节果果中的酸积累
Peipei Liu1, Chunxuan Shao1, Hang Ren1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.
International journal of biological macromolecules
|March 27, 2025
概括
一种新发现的蛋白质,MdNAC18.1,通过增加果酸的积累,增强了果果酸度. 这种转录因子直接调节参与有机酸代谢的关键基因的表达.
科学领域:
- 植物分子生物学 植物分子生物学
- 果实生物化学 果实生物化学
- 农业科学 农业科学
背景情况:
- 果酸对果的味道和新陈代谢至关重要.
- P3A-ATPase MdMa11参与调节果酸度.
研究的目的:
- 确定调节果中果酸积累的新型因素.
- 阐明MdNAC18.1影响水果酸度的分子机制.
主要方法:
- 酵母单杂交查以识别相互作用的蛋白质.
- 对转基因果,水果和西红的分析.
- 露西法酶 (LUC) 和葡萄糖化酶 (GUS) 报告测定.
- 在转基因和沉默果中进行基因表达分析.
主要成果:
- MdNAC18.1被确定为一种诱导有机酸积累的NAM域蛋白.
- MdNAC18.1直接与MdMa11促进体中的G盒动机结合,增强其表达.
- 过度表达MdNAC18.1增加,而沉默减少,包括MdWRKY126,MdMDH5,MdtDT和MdMYB1.1.在内的基因的表达.
结论:
- MdNAC18.1通过酸盐积累在调节果酸度方面发挥着重要作用.
- 这项研究揭示了一种涉及MdNAC18.1在果质量特征中的新型调节途径.
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