等离子原和等离子素诱导专门的前溶解介质,并通过5-氧基因酶促进效细胞化
Luiza O Perucci1, Fernanda S Carneiro2, Josiane C Souza2
1Department of Molecular and Cell Biology, Scripps Research, La Jolla, California, USA; Signaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
等离子原/等离子素系统增强了巨细胞中专门的前溶解媒介 (SPMs) 的产生,这是解决炎症的关键步骤. 这一过程依赖于5-氧化酶 (5-LO) 途径,以有效地进行血细胞分裂.
科学领域:
- 炎症解决生物学 炎症解决生物学
- 脂质媒介体的生物化学
背景情况:
- 血原 (Plg) / 血 (Pla) 系统是众所周知的前溶解功能,如细胞分裂.
- 专门的预溶解媒介 (SPMs) 在Plg/Pla介导的细胞分裂中的特定作用尚不清楚.
研究的目的:
- 为了确定Plg/Pla是否刺激巨细胞中SPM的产生.
- 为了研究5-氧基酶 (5-LO) 途径在Pla驱动的细胞分裂中的参与.
主要方法:
- 来自野生类型 (WT) 和Plg淘汰赛 (KO) 小鼠的骨髓衍生巨细胞 (BMDM) 用Plg/Pla.进行治疗.
- 测量了SPM产量,白血B4水平和5-,12-和15-LO的mRNA表达.
- 在体内和体外使用5-LO路径调节评估了亡性中性粒细胞的效细胞化.
主要成果:
- Plg/Pla治疗增加了素A4,马林素1和溶解素D1的产生,以及巨细胞中5,12,15-LO mRNA的表达.
- 来自Plg KO小鼠的巨细胞显示了降低的利素A4产量.
- 该药物在WT小鼠中增强了亡性中性粒细胞的效,但这种效应在5-LO淘汰小鼠中被取消,并且通过5-LO抑制显著减少.
结论:
- Plg/Pla通过在巨细胞中生成SPM来促进细胞增生.
- 5-LO通路对于Plg/Pla.的亲细胞效应至关重要.
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