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Updated: Jun 19, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

工程造型的循环导向RNA增强了基于CRISPR/Cas12f的微型基因激活和腺基编辑

Xin Zhang1,2, Mengrao Li1, Kechen Chen1

  • 1Cancer Research Institute, School of Basic Medical Sciences, State Key Laboratory of Multi-organ Injury Prevention and Treatment, Guangdong Province Key Laboratory of Immune Regulation and Immunotherapy, Southern Medical University, Guangzhou, China.

Nature communications
|March 28, 2025
PubMed
概括

工程循环导向RNA (cgRNA) 显著提高了CRISPR Cas12f基因激活和腺因基编辑效率. 这种优化对推进基因治疗应用有前途.

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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