污泥改性土壤诱导了内菌Exiguobacterium sp.中的多药物和重金属耐药性. E21L:基因组学证据的证据
Mrinmoy Patra1, Anand Kumar Pandey2, Suresh Kumar Dubey3
1Molecular Ecology Laboratory, Department of Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
World journal of microbiology & biotechnology
|March 28, 2025
概括
农业土壤中的多药耐药细菌存在风险. 来自污泥改性土壤的耐药Exiguobacterium菌株携带了抗生素和重金属耐药性基因,突显了需要更好地管理污泥,以防止抗菌素耐药性的传播.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因组学就是基因组学.
背景情况:
- 农业环境中的多药耐药细菌引发了重大公共卫生和生态问题.
- 污水污泥在农业中的应用可以将耐药微生物和耐药基因引入土壤生态系统.
研究的目的:
- 描述一种抗生素耐药的Exiguobacterium菌株,这种菌株是从在污泥改性土壤中种植的胡卜叶中分离出来的.
- 研究其基因组特征,耐药性机制以及在农业环境中传播抗菌素耐药性 (AMR) 中的潜在作用.
主要方法:
- 对Exiguobacterium sp.进行隔离和鉴定. 胡卜叶中的E21L. 胡卜叶中的E21L.
- 全基因组测序和比较基因组学.
- 抗生素耐药性,生物膜形成和压力生存的表型特征.
- 对抗微生物耐药性基因 (ARG) 和重金属耐药性基因 (HMRG) 的分析.
主要成果:
- 这种细菌是Exiguobacterium sp. E21L对多种类型的抗生素表现出高水平的耐药性 (多种抗生素耐药性指数为0.88).
- 该基因组包含许多ARG (例如,blaNDM, ermF, tetW) 和HMRG (例如, czcD, copB),以及生物膜形成和应激适应的基因.
- 该菌株在模拟的胃肠道条件下存活下来,这表明它在与宿主相关的环境中具有弹性.
结论:
- 这种细菌是Exiguobacterium sp. E21L作为农业生态系统中抗性决定因素的潜在储库.
- 调查结果强调,需要对基于污泥的肥料和改善污泥处理进行严格的监管,以减轻AMR风险.
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