使用工程Vibrio natriegens的低生物质pyruvate生产伴随着parapyruvate形成
Maurice Hädrich1,2,3, Clarissa Scheuchenegger1, Sören-Tobias Vital1
1Microbial Biotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany.
Microbial cell factories
|March 28, 2025
概括
维布里奥纳特里根被设计为使用低生物质发酵来生产高产的酸盐. 鉴定出甲基酸盐的形成是副产品,可能来自化学转化和阿尔多酶活性.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 合成生物学 合成生物学
背景情况:
- 酸盐是一种关键的工业前体,使微生物生产具有吸引力.
- 在工业应用中,Vibrio natriegens具有很高的基质吸收率.
- 本研究的重点是V. natriegens在低生物质度下用于pyruvate生产.
研究的目的:
- 为了设计Vibrio natriegens以实现高效的pyruvate生产.
- 为了优化发酵条件以获得高酸盐产量.
- 为了研究在pyruvate生物合成过程中副产品的形成.
主要方法:
- 在V. natriegens. 中,pyruvate脱酶复合物的失活.
- 通过酸盐补充,优化有氧发酵参数.
- 对副产品的形成进行分析,包括帕皮鲁瓦特.
- 基因操纵以评估HMG/CHA阿尔多酶 (LigK) 的作用.
主要成果:
- 工程V. natriegens (Δvnp12 ΔaceE) 产生了41.0g/L的酸盐,体积生产率为4.1g/L/h.
- 低生物质度 (6.6 g CDW/L) 保持在高葡萄糖吸收率 (3.5 g/g/h) 的情况下.
- 一个30%的碳不平衡被归因于parapyruvate形成,也观察到在Corynebacterium glutamicum.
结论:
- 经过代谢工程的V. natriegens在低生物量下实现了高的pyruvate生产率.
- 在酸盐生产过程中,V. natriegens和C. glutamicum都发生了对酸盐的形成.
- 甲基酸盐的形成很可能是化学甲基酸盐的转化,可能受阿尔多酶活性的影响.
相关概念视频
01:15Pyruvate Oxidation
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
01:29Fermentation
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
01:20Fates of Pyruvate
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
01:23Microbial Fermentation
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
01:28Anoxygenic Phototrophic Bacteria
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
01:26Microbes and Methanogenesis
Methanogenesis is a critical microbial process in anaerobic ecosystems responsible for the biological production of methane, a potent greenhouse gas and valuable biofuel. This metabolic pathway is primarily facilitated by methanogenic archaea, which thrive in anoxic environments such as wetlands, sediments, and animal gastrointestinal tracts. The absence of oxygen in these habitats prevents aerobic respiration, thereby favoring alternative biochemical pathways for organic matter degradation.In...