在泰国医院的高风险VREfm克隆和耐药性决定因素
Peechanika Chopjitt1, Rada Kansaen1, Sumontha Chaisaeng1
1Faculty of Public Health, Kasetsart University Chalermphrakiat, Sakon Nakhon Campus, Sakon Nakhon 47000, Thailand.
Antibiotics (Basel, Switzerland)
|March 28, 2025
概括
在泰国,抗万科胺素的Enterococcus faecium (VREfm) 血流感染主要是由携带vanA基因的ST17克隆驱动的. 大多数隔离物体对常见抗生素有耐药性,但对线化物和氨基醇仍然敏感.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 耐万科素的肠球菌 (VRE),特别是VREfm,是医疗相关感染的主要原因,特别是血液感染 (BSI).
- 了解VREfm的遗传构成和传播对于有效的控制策略至关重要.
研究的目的:
- 调查导致泰国BSI的VREfm分离物的基因型和表型特征.
- 识别序列类型 (ST),毒性因子和抗菌素耐药性概况.
主要方法:
- 多重PCR,多位序列类型化和全基因组测序 (WGS) 用于基因分析.
- 使用磁盘扩散,E-测试和微稀释进行抗生素敏感性测试.
- 微板测定用于评估生物膜形成.
主要成果:
- 所有29个VREfm分离物都具有vanA基因,并且对多种抗生素具有耐药性,包括安培,红红素,万科素和里法.
- 主要的序列类型是ST17 (72.4%),属于克隆复合体17,对西普罗夫洛克萨,tigecycline和nitrofurantoin具有广泛的耐药性.
- 所有的隔离物仍然对氨基醇和线化物敏感;检测到acm和esp等毒性基因.
结论:
- 这项研究描述了泰国侵袭性VREfm的基因组景观和克隆传播.
- 这些发现对于为感染控制措施提供信息和加强对VREfmBSI的公共卫生监测至关重要.
关键词:
肠球菌 (Enterococcus faecium) 是一个有毒的菌株.抗微生物耐药性 抗微生物耐药性细菌病的细菌病是什么意思序列类型的排序类型.这就是Vancomycin.进行全基因组测序.更多相关视频
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