在奥地利从矿井水中分离出来的细菌中的抗微生物和金属耐药性基因
Jakob Prochaska1, Heinz Reitner2, Christian Benold3
1Centre of Food Science and Veterinary Public Health, University of Veterinary Medicine, 1210 Vienna, Austria.
Antibiotics (Basel, Switzerland)
|March 28, 2025
概括
矿场细菌携带金属和抗生素耐药性基因,突出显示潜在的共同转移风险. 了解这些环境水库对于公共卫生的"One Health"方法至关重要.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 一个健康,一个健康.
背景情况:
- 矿场的微生物组是已知的抗生素耐药性基因 (ARG) 和金属耐药性基因 (MRG) 的储存库.
- 共同选择和共同转移MRG和ARG的潜力是"一个健康"框架内的关键问题.
研究的目的:
- 调查细菌金属耐药性 (MR) 和抗菌素耐药性 (AMR) 基因的同时出现和潜在联系.
- 评估从奥地利采矿场隔离的细菌中耐药基因的多样性.
主要方法:
- 从活跃和不活跃的奥地利矿场分离细菌.
- 使用MALDI-TOF和全基因组测序 (WGS) 来识别细菌物种.
- 使用分子方法检测抗微生物和金属耐药性基因.
主要成果:
- 88%的分离物携带了AMR和MR基因.
- Pseudomonadaceae 是最丰富的家族 (32.5%),经常携带mex家族的AMR基因.
- 普遍存在的MR基因包括rufB (),recGM (),copA (铜) 和mgtA (/);只有在非活性部位发现了mer基因 ().
结论:
- 来自采矿环境的细菌具有大量的MR和AMR基因负担.
- 对关键抗微生物药物的可转移性耐药性基因的存在强调了对环境监测的需要.
- 建议使用16S rRNA基因测序来准确识别各种采矿微生物组中的物种.
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