来自本土益生菌菌株的后生物基提取物:食品保存和抗菌防御的有希望的战略
Diana Molina1, Ioana C Marinas2, Evelyn Angamarca1
1Biofood and Nutraceutics Research and Development Group, Faculty of Engineering in Agricultural and Environmental Sciences, Universidad Técnica del Norte, Av. 17 de Julio s-21 y José María Córdova, Ibarra 100150, Ecuador.
Antibiotics (Basel, Switzerland)
|March 28, 2025
概括
来自乳酸细菌的后生物基提取物 (PBEs) 显示出作为天然食品防腐剂的潜力. 两种配方,FU6和FU13,有效抑制有害细菌,并表现出无毒的抗氧化特性.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 食品腐烂和安全是与致病微生物相关的主要问题.
- 来自乳酸细菌 (LAB) 的后生物质为食品保存提供了一个有希望的替代方案.
- 这项研究侧重于基于后生物的提取物 (PBEs),包括无细胞超物 (CFS) 和外聚糖化物 (EPS) 分数.
研究的目的:
- 研究来自本地益生菌菌株的PBEs的生物和物理化学特性.
- 评估PBEs的抗菌活性,抗氧化能力和细胞毒性.
- 为了描述有前途的PBE配方的组成.
主要方法:
- 对抗多抗药性大肠杆菌 (Escherichia coli) 的抗菌活性进行了测试.
- 进行了抗氧化能力和细胞毒性测试.
- 显微镜 (TEM,SEM),ATR-FTIR和1H-NMR光谱用于表征.
主要成果:
- FU6 (CFS UTNGt28:EPS UTNGt2) 和FU13 (CFS UTNGt21O) 显示出强烈的活动.
- 显微镜证实了剂量和时间依赖的细菌膜和细胞壁损伤.
- FU6显示出卓越的抗氧化活性;FU6和FU13都显示出细胞特异性反应,并含有蛋白质,脂质,碳水化合物,核酸和关键代谢物.
结论:
- 在非缩剂量下,FU6和FU13是有前途的安全有效的后生物制剂.
- 需要进一步的研究才能充分阐明它们的分子组成.
- 在食品防腐和制药发展方面的潜在应用需要进一步研究.
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