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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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相关实验视频

Updated: Jun 30, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

检测mRNA转录变种的检测

Kevin Vo1, Sharmin Shila1, Yashica Sharma1

  • 1Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA.

Genes
|March 28, 2025
PubMed
概括
此摘要是机器生成的。

细胞基因通过复杂的处理产生多个转录变异. 了解这些变异是基因调节的关键,新的RNA测序方法可以改善它们的检测.

关键词:
有关RNA测序的RNA测序对NGS数据的分析.基因表达的基因表达方式mRNA转录变体的变体空间转录学 空间转录学对NGS数据的验证.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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相关实验视频

Last Updated: Jun 30, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 大多数真核基因产生多个成熟的信使RNA (mRNA) 分子,称为转录变异.
  • 这些变异源于替代转录起点和转录后处理等机制.
  • 转录变异可以产生具有不同功能的蛋白质或非编码RNA,影响细胞过程.

研究的目的:

  • 审查检测转录变异的分子技术的进步.
  • 突出研究转录多样性的生物学意义和挑战.
  • 引导研究人员选择有效的转录变异检测方法.

主要方法:

  • 传统方法的审查:RT-PCR,RT-qPCR,RACE-PCR,北方涂抹,RNase保护测定和微阵列.
  • 专注于RNA测序 (RNA-Seq) 作为一种强大的技术,用于识别已知的和新的转录变异.
  • 讨论影响RNA-Seq有效性的因素,包括测序方法和数据分析.

主要成果:

  • 传统方法对于检测已知的转录变体是有效的.
  • RNA测序为识别新型转录变异提供了卓越的能力.
  • 技术的选择影响了转录变异检测的精度和范围.

结论:

  • 准确检测转录变异对于理解基因调节至关重要.
  • 分子技术的进步,特别是RNA-Seq,增强了对转录物多样性的研究.
  • 有效使用这些技术有助于理解转录变异在各种生物环境中的影响.