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Updated: Jun 29, 2026

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来自Thermococcus kodakarensis的一种双功能糖突变酶/糖突变酶:生物物理分析和冷EM结构

Zahra Naz1,2, Ishan Rathore2, Muhammad Saleem1

  • 1School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan.

Biomolecules
|March 28, 2025
PubMed
概括

研究人员特征了一种来自Thermococcus kodakarensis的新型酶,具有糖突变酶 (PGM) 和胺突变酶 (PMM) 活动. 这是一种双功能酶.

关键词:
热球菌 (Thermococcus kodakarensis) 是一种可怕的病毒.低温电子显微镜 (cryo-EM) 的使用糖转基因酶/糖转基因酶/糖转基因酶这是一个三维结构结构.

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Cryo-electron Microscopy01:28

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...