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染色体层次的基因组组合和对比转录组分析确定了节能作为Hilsa Shad,Tenualosa ilisha (Clupeiformes:Dorosomatidae) 无适应的关键策略
Kishor Kumar Sarker1,2, Liang Lu1,2, Roland Nathan Mandal3
1Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, Shanghai 201306, China.
Biomolecules
|March 28, 2025
概括
一种无人种的鱼Tenualosa ilisha的基因组揭示了迁徙的分子适应性. 肝脏和肌肉中的关键基因扩张和代谢转变在具有挑战性的河流旅程中支持能量需求和度调节.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 鱼类生理学 鱼类生理学
背景情况:
- 无体迁移对像Tenualosa ilisha这样的鱼类来说是生理上要求很高的.
- 了解迁移的分子基础对于保护和水产养殖至关重要.
研究的目的:
- 为了生成Tenualosa ilisha的染色体级基因组.
- 为了研究迁移期间肝脏和肌肉的转录变化.
- 确定基底的分子机制适应不同的环境.
主要方法:
- 染色体层次的基因组组装Tenualosa ilisha. 这是一个很好的例子.
- 在海洋和淡水环境之间,肝脏和肌肉组织的比较转录学.
- 基因家族扩张分析和选择压力评估.
- 共同表达网络分析.
主要成果:
- 确定了用于信号和度调节的快速基因家族扩张.
- 在环境之间检测到肝脏 (1298个转录) 和肌肉 (252个转录) 中显著的基因表达差异.
- 假设的肌肉蛋白质转化会通过乌比奎丁-蛋白质体复合体为迁移能量提供燃料.
- 观察到增强脂肪酸合成和调节FADS2/ELOVL2在河流肝脏的能量利用.
- 发现了活性葡萄糖生成和肝脏减少胰岛素信号的证据,表明适应饥饿.
结论:
- 鱼基因组为进化和功能基因组学提供了宝贵的资源.
- 基因表达,新陈代谢和信号传递中的分子适应对于成功的无动物迁移至关重要.
- 肝脏和肌肉的生理变化突出显示了特定器官对迁移挑战的适应.
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