相关实验视频
Updated: Jul 31, 2026

07:19
High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
Published on: February 2, 2011
化学修饰与基于CRISPR的同热测试相结合,用于敏感检测DNA氧甲基化
Guangrong Zou1, Penglong Si2, Jiaqi Wang2
1Department of Clinical Laboratory, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong 518107, P. R. China.
ACS sensors
|March 28, 2025
概括
研究人员开发了一种敏感的方法来检测5-Hydroxymethylcytosine (5hmC),这是一种与癌症等疾病相关的重要表观遗传标记. 这种新技术允许精确量化生物样本中的5hmC,即使在微量水平.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 5-基甲基细胞氨酸 (5hmC) 是一种关键的表观遗传标记物,参与DNA脱甲基和疾病进展.
- 在复杂的生物样本中检测和量化5hmC的灵敏和方便的方法目前有限.
- 5hmC与各种疾病有关,包括糖尿病,结直肠癌和肝癌.
研究的目的:
- 开发一种新的,过敏的和定量方法来检测5-氧甲基细胞素 (5hmC).
- 为了使复杂的生物环境中微量检测到5hmC.
主要方法:
- 使用多功能光敏感探头进行5hmC-DNA的特定标记,丰富和化.
- 整合了带有滚动圆放大和Cas12a的异热分析,以准确识别5hmC.
- 在各种生物样本中使用实时PCR进行全球5hmC量化.
主要成果:
- 在低至11 fM的微量中实现了5hmC DNA的定量检测.
- 在各种生物样本中成功测量了全球5hmC,只使用了10 ng的输入DNA.
- 该方法显示没有序列限制,突出其广泛的适用性.
结论:
- 开发的方法提供了一个高度敏感和定量方法来检测5hmC.
- 这种技术显示出分析血液,尿液和唾液等复杂样本中微量核酸的修饰基的巨大潜力.
- 该方法为疾病生物标志物研究和诊断提供了宝贵的工具.
相关概念视频
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

