多草药配方对肝脏健康的毒性分析:急性和亚急性评估
Kalyani A Autade1,2, Ramdas B Pandhare3
1Department of Pharmacology, Savitribai Phule Pune University (SPPU), Progressive Education Society's Modern College of Pharmacy, Sector-21, Yamunanagar, Pune. MH Nigdi-411044, India.
Toxicology research
|March 28, 2025
概括
这项研究发现一种多草药配方是安全的消费. 在老鼠中进行的毒性测试没有显示任何不良影响,表明其在市场上发布的安全性.
科学领域:
- 药理学和毒理学 药理学和毒理学
- 自然产品化学 自然产品化学
- 药物安全评估 药物安全评估
背景情况:
- 全天然产品在上市之前需要严格的安全评估.
- 多草药配方 (PHF) 越来越受欢迎,但需要毒理学验证.
- 来自自然来源的肝护剂需要进行彻底的安全分析.
研究的目的:
- 评估具有肝脏保护性质的特定多草药配方 (PHF) 的毒理特征.
- 为了评估PHF在Wistar Albino大鼠中的急性,次急性和次慢性毒性.
- 根据国际指南,确定PHF的安全性和潜在的器官毒性.
主要方法:
- 根据经合组织指南423进行的急性毒性测试,单次口服剂量为2000mg/kg.
- 亚急性毒性测试遵循经合组织指导方针407,重复口服剂量为50,100和200毫克/公斤,持续28天.
- 评估包括临床观察,生物化学测定 (AST,ALT,脂质概况),血液学和重要器官的组织病理学.
主要成果:
- 急性毒性研究:在2000 mg/kg以下没有观察到死亡率,毒性征兆或行为异常,表明LD50大于2000 mg/mL.
- 亚急性毒性研究:治疗组与对照组之间没有显著的生物化学,血液学或组织学差异 (P <0.05).
- 组织病理学检查显示器官结构正常,关键器官 (心脏,大脑,脏,肝脏,肺部,脏) 没有退行性变化.
结论:
- 多草药配方 (PHF) 在急性和亚急性研究中显示出良好的安全性,没有观察到毒性.
- 肝功能标志物 (AST,ALT) 或脂质配置文件中PHF不会引起显著的变化.
- 组织病理学发现证实没有主要的器官相关毒性,支持这种PHF的安全使用.
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