在心脏手术期间对中性粒细胞活性进行多式监测
Darko Jovanovski1, Lisa Wohlgemuth2, Pascal Max Lucien Lessing2
1Department of Cardiothoracic and Vascular Surgery, University Hospital Ulm, Ulm, Germany.
Frontiers in immunology
|March 28, 2025
概括
心脏手术会触发早期的中性粒细胞激活,在标准炎症标志物之前. 中性粒细胞功能和血小板-中性粒细胞复合体在手术期间和手术后发生变化,可能会影响免疫功能.
科学领域:
- 免疫学 免疫学 免疫学
- 心血管外科心血管外科
- 炎症研究 炎症研究
背景情况:
- 心脏手术,特别是带有体外循环的大动脉手术,会诱导缺血-再输血损伤.
- 这种损伤会引发炎症反应,可能导致器官损伤,延长住院时间和增加死亡率.
研究的目的:
- 在接受大动脉门手术的患者中全面监测与中性粒细胞相关的炎症和细胞变化.
- 在手术期间和之后分析中性粒细胞生理学,表型和功能的变化.
主要方法:
- 使用流细胞计,ELISA和显微镜分析中性粒细胞激活和功能.
- 评估了特定的标记物,如CD11b,CD66b,CD10,以及血小板-中性粒细胞复合体的形成.
- 评估了对血小板激活因子 (PAF) 的反应,以及反应性氧物种生成和细胞活性.
主要成果:
- 在手术期间观察到中性粒细胞的激活,C-反应性蛋白和介质素-6.6的增加之前.
- 中性粒细胞对PAF的反应改变了手术前和手术后的CD10表达和脱极化.
- 血小板-中性粒细胞复合体的形成在手术期间受损,但在术后正常化.
结论:
- 在手术期间的中性粒细胞激活和经手术期间/术后的中性粒细胞功能变化表明了暂时的免疫功能障碍.
- 中性粒细胞和血小板活动的变化可能会增加对感染性或静血并发症的易感性.
- 需要进一步的研究,以了解这些观察到的免疫变化的临床影响.
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