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长非编码RNAMIR210HG在高血压中的诊断价值和调节作用
Wenxin Zhao1, Yajing Zheng2, Yuanyuan Su3
1Department of Cardiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
Minerva cardiology and angiology
|March 28, 2025
概括
上调的MIR210HG是一种有前途的高血压诊断生物标志物. 这种长长的非编码RNA在高血压模型中影响细胞增殖,迁移和细胞亡,可能准miR-125b-5p.
科学领域:
- 心血管生物学 心血管生物学
- 分子遗传学 分子遗传学
- 生物标志物发现发现
背景情况:
- 高血压是一个全球性的健康问题,治疗和控制率很低.
- 长非编码RNAMIR210HG在高血压中的作用仍然在很大程度上未被描述.
- 研究新的分子机制对于改善高血压管理至关重要.
研究的目的:
- 为了研究MIR210HG在高血压中的表达和功能.
- 评估MIR210HG作为高血压的潜在诊断生物标志物.
- 探索MIR210HG及其下游目标miR-125b-5p在高血压中的关系.
主要方法:
- qRT-PCR用于量化患者和细胞模型中的MIR210HG和miR-125b-5p表达.
- 进行ROC曲线分析,以评估MIR210HG.的诊断性能.
- 细胞测试 (CCK-8,Transwell,流动细胞计) 来评估增殖,迁移和亡.
- 双 luciferase 记者测定以确认 MIR210HG 和 miR-125b-5p 之间的相互作用.
主要成果:
- 在高血压患者中,MIR210HG显著上调,与疾病严重程度有积极的相关性.
- 作为高血压的诊断生物标志物,MIR210HG显示出高灵敏度 (81.93%) 和特异性 (83.28%) (AUC=0.8765).
- 在体外,MIR210HG的敲除逆转了高血压引起的细胞增殖和迁移的减少,并抑制了细胞亡和炎症.
结论:
- 升级的MIR210HG作为一个有前途的诊断生物标志物用于识别高血压.
- MIR210HG影响高血压中的关键细胞过程,包括增殖,迁移,亡和炎症.
- 在人类静脉内皮细胞中,MiR-125b-5p被确定为MIR210HG的潜在下游标.
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