乳酸细菌向NF-κB信号传递,以缓解胃炎症
Shiying Wu1, Yuenuo Luo1, Fangtong Wei1
1Key Laboratory for Food Microbial Technology of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, China. wushiying@zjgsu.edu.cn.
乳植物菌植物ZJ316 (L. plantarum ZJ316) 减少了由Helicobacter pylori (H. pylori) 感染引起的炎症. 这种益生菌抑制NF-κB通路并改善肠道微生物群,为H. pylori保护提供潜在的功能性食物.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 杆菌感染是胃炎和癌症的主要原因.
- 益生菌显示出抗炎潜力,但它们对H. pylori的机制尚未完全理解.
- 乳植物菌植物ZJ316 (L. plantarum ZJ316) 之前已经在体内表现出对H. pylori的抗炎作用.
研究的目的:
- 阐明L. plantarum ZJ316抑制H. pylori诱导的胃炎症的机制.
- 评估L. plantarum ZJ316对炎症信号通路和胃微生物群组成的影响.
主要方法:
- 在用L. plantarum ZJ316.6治疗的H. pylori感染的AGS细胞中评估了促炎性细胞因子表达.
- 研究了L. plantarum ZJ316对NF-κB信号通路 (IκBα降解,p65酸化,核转位) 的影响.
- 给 H. pylori 感染的小鼠注射了 L. plantarum ZJ316,并通过转基因组学分析了血清炎症标志物和胃微生物群组成.
主要成果:
- L. plantarum ZJ316在感染细胞中显著降低了促炎性细胞因子.
- 益生菌通过防止IκBα降解和p65酸化/转移来抑制NF-κB信号传递.
- 在体内,L. plantarum ZJ316缓解了炎症,降低了TNF-α和PGII,增加了IL-10,并调节了胃微生物群,减少了Prevotella和Desulfovibrio,同时增加了Ligilactobacillus和Akkermansia.
结论:
- L. plantarum ZJ316对H. pylori感染具有显著的抗炎作用.
- 该机制涉及抑制NF-κB信号通路.
- L. plantarum ZJ316调节胃微生物群,支持其作为H. pylori相关疾病的功能性食物的作用.
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