在种子原始化过程中揭示了种子储存的mRNA的动态
Patricija Gran1, Tessa W Visscher1, Bing Bai1
1Wageningen Seed Science Centre, Laboratory of Plant Physiology, Wageningen University, 6708 PB, Wageningen, the Netherlands.
The New phytologist
|March 28, 2025
概括
种子初始化通过准备mRNA以更快的翻译来提高发芽速度. 然而,这种播种前处理减少了种子的寿命,并改变了与压力相关的基因表达.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 种子生理学 种子生理学
背景情况:
- 种子初始化是一种播种前的处理,可以提高发芽效率和均性.
- 尽管有好处,但种子初始化可能会对种子的寿命产生负面影响.
- 了解初始化过程中的分子变化对于优化种子处理至关重要.
研究的目的:
- 为了研究水原化过程中的mRNA动态和转原子变化.
- 为了确定分子机制,负责加速发芽后的原始化.
- 为了探索水原化对种子转录组的影响.
主要方法:
- 用多体形分析分析了在各种水原化阶段的mRNA-ribosome复合体.
- 核糖体新生链复杂测序 (RNC-seq) 用于评估转录和转录的变化.
- 基因表达在原始种子和干种子中的比较分析.
主要成果:
- 与干燥种子相比,含水的种子表现出更多的mRNA-ribosome关联.
- 这表明,与发芽相关的mRNA与核糖体进行了预先关联,以便快速翻译.
- 原始化导致与压力相关的转录的丰富性减少.
结论:
- 水原化加速发芽,通过预先将mRNA与核糖体结合在一起,从而在重新嵌合时实现更快的翻译.
- 观察到的mRNA动态和转录组的变化有助于改善发芽率.
- 进一步的研究可以确定特定的基因参与增强的发芽后化.
关键词:
阿拉比多普西斯塔利亚纳.在RNC-seqq.发芽 发芽 发芽 是一个过程.液压制是指使用水性制.长寿 长寿是一个问题.的mRNA动态.多组体概况分析 (polysome profiling) 是一种多组体概况分析方法.种子 种子 种子更多相关视频
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相关概念视频
02:01pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
02:41Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
02:33Seed Structure and Early Development of the Sporophyte
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.