从土耳其传统酸奶中分离出来的15种Streptococcus thermophilus菌株的比较基因组分析
Deniz Kiraz1, Ali Özcan2,3
1Animal Originated Foodstuffs Department, Central Research Institute of Food and Feed Control, Bursa, Turkey. deniz.kiraz@tarimorman.gov.tr.
Antonie van Leeuwenhoek
|March 28, 2025
概括
与工业菌株相比,传统的酸奶细菌Streptococcus thermophilus表现出独特的基因组特征和增强的碳水化合物代谢. 这些发现揭示了传统发酵的适应性和潜在的健康益处,有助于初始化培养的发展.
科学领域:
- 微生物学和基因组学 微生物学和基因组学
- 食品科学与技术 食品科学与技术
- 乳制品发酵 乳制品发酵
背景情况:
- 热菌 (Streptococcus thermophilus) 在酸奶发酵过程中至关重要.
- 与工业产品相比,传统发酵产品的菌株研究不足.
- 了解基因组多样性是优化初始培养的关键.
研究的目的:
- 描述土耳其传统酸奶中S. thermophilus菌株的基因组多样性和功能潜力.
- 将这些传统品种与工业品种进行比较.
- 为了确定传统发酵环境的遗传适应性.
主要方法:
- 15种传统的S. thermophilus菌株的全基因组测序.
- 先进的生物信息学分析,包括泛基因组分析和SNP分析.
- 与工业S. thermophilus菌株进行比较的基因组学.
主要成果:
- 传统菌株表现出明显的家族遗传模式和显著的功能多样性,尽管高基因组均性.
- 鉴定了丰富的碳水化合物活性酶 (CAZyme) 谱和广泛的GABA生物合成途径.
- 基因组岛屿,多样化的外聚糖,细菌素基因和CRISPR-Cas系统是普遍存在的.
结论:
- 基因组可塑性使S. thermophilus能够适应传统的酸奶环境.
- 传统菌株具有独特的遗传特征,包括增强的碳水化合物代谢和潜在的健康促进性质.
- 研究结果为开发新型初始培养和保持传统乳制品品质提供了洞察力.
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