葡萄糖改变了对尿病原菌大肠杆菌 gentamicin 的进化反应
Shalini Choudhary1, Jacob A Smith1, Alan McNally1
1Department of Microbes, Infection and Microbiomes, School of Infection, Inflammation and Immunology, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK.
Microbiology (Reading, England)
|March 28, 2025
概括
暴露于葡萄糖可以改变尿病原性大肠杆菌 (UPEC) 抗生素耐药性的演变,这是尿路感染 (UTI) 的常见原因. 这一发现可能会为糖尿病患者的尿路感染治疗提供信息.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 传染性疾病 传染性疾病
背景情况:
- 尿路感染 (UTI) 是一个重要的健康和经济负担,尿病原性大肠杆菌 (UPEC) 是主要的致病原体.
- 抗生素耐药性UPEC的增加需要了解影响其演变和治疗疗效的因素.
- 糖尿病,标志着糖尿症,是已知的尿路感染风险因素,与更严重的感染有关,但葡萄糖在UPEC演变中的作用仍然不清楚.
研究的目的:
- 调查葡萄糖暴露对UPEC演变的影响.
- 确定葡萄糖如何影响UPEC对抗生素治疗的反应,特别是 gentamicin.
- 在特定的环境条件下识别UPEC中的遗传突变.
主要方法:
- 在不同的条件下,单个UPEC分离物的实验演变:葡萄糖暴露, gentamicin 暴露和结合葡萄糖-gentamicin 暴露.
- 基因组分析以确定赋予耐药性或适应性的突变.
- 对不同实验组的突变频率进行比较分析.
主要成果:
- 长期暴露于葡萄糖导致RNA聚合酶西格玛因子基因的突变,rpoS.
- 甘他治疗诱导了trkH (吸收) 和hyxB (自传递物) 等基因的突变.
- 值得注意的是,在暴露于葡萄糖和 gentamicin 的 UPEC 种群中,这些 gentamicin 诱导的突变都不存在,这表明葡萄糖具有保护性或调节性的作用.
结论:
- 葡萄糖可能会影响在抗生素压力下UPEC突变的生存和进化.
- 这些发现表明,代谢因素,如葡萄糖的可用性,可以改变UPEC的进化轨迹,以应对抗生素.
- 这项研究为UPEC的演变提供了新的见解,并为糖尿病患者等高风险人群的尿路感染提供了潜在的治疗策略.
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