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来自人类支气管上皮细胞的微囊调节巨细胞在感染期间的激活Mycobacterium abscessus

Carlyn M Guthrie^1,2, Amber C Meeker^1,2, Ashton E Self^1,2

¹Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, United States.

Journal of proteome research

|March 28, 2025

概括

人类呼吸道细胞在非结核性真菌菌 (NTM) 感染时释放微. 这些微粒细胞激活M2巨细胞,影响宿主免疫力和细菌生存.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
微生物学微生物学

背景情况:

细胞间通信对于宿主免疫力对抗细菌感染至关重要.
非结核菌 (NTM) 和 Mycobacterium abscessus (M. ab) 一样,会导致严重的肺部感染,特别是在囊性纤维化 (CF) 和 COPD 患者中.
在NTM感染期间,在细胞水平上了解宿主-病原体相互作用是有限的.

研究的目的:

描述由M. ab感染的人类支气管上皮细胞释放的微 (MVs).
研究这些MVs对巨细胞激活和极化的影响.
阐明MVs在宿主免疫反应中对M. ab感染的作用.

主要方法:

从M. ab感染和未感染的人类支气管上皮细胞中分离和表征微粒细胞.
在细胞培养中评估MV对THP-1衍生的巨细胞激活和极化的影响.
单独的MVs的蛋白质组分析,以确定富含的蛋白质和途径.

主要成果:

与未感染细胞的MV相比,来自M. ab感染细胞的MV促进了M2巨细胞的激活.
蛋白质组分析显示,受感染细胞的MV中细胞粘附通路蛋白的丰富.
在MV上的细胞间粘附分子1 (ICAM-1) 调节了它们被巨细胞吸收.

结论:

人类呼吸道上皮细胞释放MVs作为对M. ab感染的反应.
这些MV调节巨细胞激活,影响宿主的免疫反应.
这些发现表明,在NTM感染期间,呼吸道上皮细胞与免疫细胞进行通信的新机制.

关键词:

这种细菌是Mycobacterium abscessus.皮质细胞是表皮细胞.细胞外囊泡中的细胞外囊泡.细胞间通信是细胞间的通信.巨细胞是什么?巨细胞是什么?微小的微小的微小的微小的微小蛋白质组学蛋白质组学

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